长顺绿壳蛋鸡ITPR1基因RYDR-ITPR结构域的多态性对蛋壳品质的影响

Effects of Polymorphism of the RYDR-ITPR Domain of ITPR1 Gene on Eggshell Quality in Changshun Green-eggshell Chicken

为了探究贵州特产长顺绿壳蛋鸡ITPR1基因RYDR-ITPR结构域的多态性对蛋壳品质的影响,采用在线引物设计软件Primer Premier 3.0设计1对引物,扩增ITPR1基因RYDR-ITPR结构域,利用双向直接测序和序列比对法挖掘单核苷酸多态性(SNP)位点,应用SPSS 18.0软件分析SNP位点对长顺绿壳蛋鸡蛋壳品质的影响。结果显示,在ITPR1基因第17~19内含子区共发现3个SNP位点,分别为位于18内含子上的g.18853584 G>T、g.18853600 A>G突变位点和19外显子上的g.18853848 C>T突变位点;3个SNP位点均为中度多态,均未偏离Hardy-Weinberg平衡(P>0.05),共检测到4种单倍型和7种双倍型。关联分析结果显示,位于19外显子上的g.18853848 C>T突变对蛋壳厚度有显著影响,TT、CC基因型显著高于TC基因型(P<0.05);双倍型H3H3的蛋壳厚度和蛋壳强度测定值最高。综上,长顺绿壳蛋鸡ITPR1基因RYDR-ITPR结构域中g.18853848 C>T突变可能是影响蛋壳厚度的标记位点,双倍型H3H3可能是蛋壳厚度和蛋壳强度的有利双倍型。

In order to investigate the effect of polymorphism of ITPR1 gene RYDR-ITPR domain on eggshell quality in Changshun green-eggshell chicken of Guizhou Province.One pair of primers were designed using online Primer premier 3.0 software to amplify the ITPR1 gene RYDR-ITPR domain.Single nucleotide polymorphism(SNP)was excavated by bidirectional direct sequencing and sequence comparison method.SPSS 18.0 software was used to analyze the influence of SNP sites on the eggshell quality of Changshun green-eggshell chicken.The results showed that three SNP sites were found in the 17th—19th introns of ITPR1 gene,which were g.18853584 G>T and g.18853600 A>G on the 18th intron,and g.18853848 C>T on the 19th exon.The three SNP sites were all moderately polymorphic and did not deviate from the Hardy-Weinberg equilibrium(P>0.05).Four haplotypes and seven diplotypes were detected at three SNP sites.The results of correlation analysis showed that the mutation g.18853848 C>T on the 19th exon had a significant effect on eggshell thickness,and TT and CC genotypes were significantly higher than genotype TC(P<0.05).The shell thickness and shell strength of diplotype H3H3 were the highest.To sum up,the mutation g.18853848 C>T in the RYDR-ITPR domain of the ITPR1 gene in Changshun green-eggshell chicken may be a marker site that affects the thickness of eggshell,and diplotype H3H3 may be a favorable type for thickness and strength of eggshell.