全蝎软膏对大鼠糖尿病皮肤溃疡创面愈合的效果评价

Effect of Scorpion Ointment on the Wound Healing of Diabetic Cutaneous Ulcer in Rats

目的:探讨全蝎软膏治疗糖尿病皮肤创面愈合的作用及其机制。方法:采用SPF级SD大鼠构建糖尿病皮肤溃疡模型,将其随机平均分为对照组、模型组、全蝎软膏组和湿润烧伤膏组。造模后,分别在创面处涂抹全蝎软膏或湿润烧伤膏处理,空白组和模型组用PBS处理,观察和比较各组创面的愈合情况,并收集用药后3 d、7 d、10 d和14 d的创面肉芽组织进行HE染色,通过ELISA及荧光定量PCR检测肉芽组织内碱性成纤维细胞生长因子(bFGF)、血管性假血友病因子(v WF)、肿瘤坏死因子α(TNF-α)与Smad 4基因的表达。结果:成功构建大鼠糖尿病皮肤溃疡模型,成模率为96.15%。创伤后第3 d、7 d、10 d和14 d,各治疗组创面愈合率均显著高于模型组(P<0.05),给药14 d时,全蝎软膏组创面愈合率已接近对照组水平。HE染色结果显示与模型组相比,全蝎软膏组中肉芽组织生长较快、毛细血管数量增多且组织纤维化程度较低。ELISA和荧光定量PCR检测结果显示与模型组和湿润烧伤膏组相比,全蝎软膏组肉芽组织内bFGF、v WF含量上调更显著(P<0.05),而TNF-α含量与Smad 4 m RNA显著降低(P<0.05)。结论:全蝎软膏可促进糖尿病皮肤溃疡创面肉芽组织的生长,可能与其抑制炎症反应并改善血管功能有关。

Objective: To explore the effect and mechanism of scorpion ointment in the treatment of diabetic cutaneous ulcer.Methods: The diabetic cutaneous ulcers model was established with SPF grade SD rats and divided into control group, model group,scorpion ointment group and MEBO group, respectively. After modeling, the scorpion ointment or MEBO were applied to the wound surface, while the control group and model group were treated with PBS. Observation and comparison of wound healing in each group,and the granulation tissue of wound was collected for H.E staining. ELISA and real-time PCR detection for the expression of basic fibroblast growth factor(bFGF), vascular pseudohemophilia factor(VWF), tumor necrosis factor-α(TNF-α) and Smad-4 in granulation tissue on the 3 d, 7 d, 10 d and 14 d after treatment. Results: The model of diabetic cutaneous ulcer in rats was established successfully and the model rate was 96.15%. On the 3 d, 7 d, 10 d and 14 d after trauma, the wound healing rate of each treatment group was higher than that of the model group(P<0.05). The wound healing rate of scorpion ointment group was close to that of the control group at the 14 d of administration. The results of H.E staining showed that the granulation tissue grew faster, the number of capillaries increased and the degree of tissue fibrosis was lower in the scorpion ointment group than in the model group. The results of ELISA and qPCR showed that bFGF and v WF in granulation tissue of the scorpion ointment group were significantly higher than those of the model group and MEBO group(P<0.05), while the TNF-α and Smad 4 gene decreased significantly(P<0.05). However, the therapeutic effect of scorpion ointment is more significant. Conclusion: The scorpion ointment can promote the growth of granulation tissue in diabetic cutaneous ulcer, which may be related to its inhibition of inflammation and improvement of vascular function.