紫外诱变结合链霉素抗性选育小诺霉素高产菌株

Breeding the High-yield Strain of Micronomicin by UV Irradiation Mutagenesis and Streptomycin-resistant Screening

以自然筛选的小诺霉素产生菌XDBJ-CF为诱变选育的出发菌株,经最适照射剂量60 s的紫外诱变后,在含20μg/mL链霉素的高氏平板上培养,获得链霉素抗性突变株。突变株摇瓶初筛试验显示,高产突变株的数量大于低产突变株。从正突变株中挑选相对发酵单位130%以上的16株高产突变株进行摇瓶复筛,获得2株小诺霉素高产突变株XDBJ-CF-09-24、XDBJ-CF-09-90,其小诺霉素产量分别比出发菌株提高37.8%和46.3%,C2b组分分别较出发菌株提高10%和20%。

The original strain XDBJ-CF of micronomicin is obtained from the environment.It was irradiated by UV light for 60 s,then cultured in the plates with streptomycin of 20μg/mL.The mutants with streptomycin-resistant was obtained and fermented in the flasks.The result showed that among all flasks,the amount of the mutants with high yield of micronomicin was higher than those with low yield of micronomicin.We selected the 16 mutants with fermentation unit higher than 130%to ferment.Two mutants with the high yield of micronomicin were obtained:XDBJ-CF-09-24 and XDBJ-CF-09-90.The micronomicin yields generated from two mutants,XDBJ-CF-09-24 and XDBJ-CF-09-90 were 37.8%and 46.3%higher,respectively,and the component C2 b content of the production was 10%and 20%higher than those from the original strain.