胆管癌组织白介素-6、环氧合酶-2和血管内皮生长因子的表达及临床意义

Expression and Clinical Significance of IL-6,COX-2 and VEGF in Cholangiocarcinoma

目的:探讨胆管癌组织白介素-6(IL-6)、环氧合酶-2(COX-2)和血管内皮生长因子(VEGF)的表达及临床意义。方法:将手术切除并经病理诊断确诊的胆管癌石蜡包埋标本80例纳为胆管癌组,另取癌旁正常胆管组织作为对照组,采用免疫组织化学SP法检测两组组织中IL-6、COX-2、VEGF的表达情况并做比较,分析胆管癌组织中VEGF、COX-2、IL-6阳性表达与临床病理特征关系,采用Spearman等级相关分析胆管癌组织中VEGF、COX-2、IL-6表达的相关性。结果:胆管癌组的VEGF、COX-2、IL-6阳性表达率均显著高于对照组,组间比较差异有统计学意义(P<0.05)。胆管癌组织中VEGF、COX-2、IL-6阳性表达率与有无淋巴结转移、TNM分期、分化程度有关(P<0.05),而与性别、年龄、肿瘤直径无关(P>0.05),其中有淋巴结转移、TNM分期Ⅲ~Ⅳ期、低分化程度的胆管癌患者的VEGF、COX-2、IL-6阳性表达率高于无淋巴结转移、TNM分期Ⅰ~Ⅱ期、中高分化程度的胆管癌患者(P<0.05)。Spearman等级相关分析显示,胆管癌组织中VEGF与COX-2、IL-6呈正相关(P<0.05),COX-2与IL-6也呈正相关(P<0.05)。结论:胆管癌组织IL-6、COX-2、VEGF均呈现高表达,并与胆管癌的生长、转移密切相关,检测IL-6、COX-2和VEGF有助于判断胆管癌疾病进展。

Objective:To explore the expression and clinical significance of interleukin-6(IL-6),cyclooxygenase-2(COX-2)and vascular endothelial growth factor(VEGF)in cholangiocarcinoma.Methods:80 patients with paraffin-embedded specimens of cholangiocarcinoma surgically resected and pathologically diagnosed were selected as cholangiocarcinoma group,and the normal bile duct tissues adjacent to cancer as control group.The expressions of IL-6,COX-2 and VEGF in the two groups were detected by immunohistochemical SP method and compared.The positive expressions of VEGF,COX-2 and IL-6 in cholangiocarcinoma tissues were analyzed and compared with clinical data.Spearman grade correlation was used to analyze the correlation of expression of VEGF,COX-2 and IL-6 in cholangiocarcinoma.Results:The positive expression rates of VEGF,COX-2 and IL-6 in cholangiocarcinoma group were significantly higher than those in control group,and there was significant difference between the two groups(P<0.05).The positive expression rates of VEGF,COX-2 and IL-6 in cholangiocarcinoma tissues were correlated with lymph node metastasis,TNM stage and differentiation degree(P<0.05),but not with sex,age and tumor diameter(P>0.05).The positive expression rates of VEGF,COX-2 and IL-6 in cholangiocarcinoma tissues were correlated with lymph node metastasis,TNM stage and differentiation degree(P<0.05).Spearman grade correlation analysis showed that there was a positive correlation between the positive expression rate of VEGF and COX-2,IL-6 in cholangiocarcinoma(P<0.05),and the positive expression rate of COX-2 and IL-6 in cholangiocarcinoma(P<0.05).Conclusion:The IL-6,COX-2 and VEGF are highly express in cholangiocarcinoma tissues,which are closely related to the growth and metastasis of cholangiocarcinoma.Detection of IL-6,COX-2 and VEGF is helpful in judging the progress of cholangiocarcinoma.