枸杞瘿螨致瘿后宁夏枸杞叶片代谢组学分析

Metabolomics analysis of Lycium barbarum leaves after Aceria pallida infestation

枸杞瘿螨Aceria pallida是我国枸杞主产区最常见的成灾害虫之一,取食为害枸杞叶片导致局部组织畸形膨大形成虫瘿,严重影响枸杞生长和产量,然而致瘿过程对枸杞初生代谢和次生代谢的影响却知之甚少。该文通过实验条件下人工接种枸杞瘿螨,利用LC-MS/MS技术对枸杞瘿螨致瘿后宁夏枸杞叶片进行代谢组学测定,比对分析枸杞瘿螨致瘿后枸杞叶片初生和次生代谢产物的变化,为枸杞虫瘿叶片综合开发利用提供参考。结果显示,枸杞叶片富含氨基酸类和黄酮类物质,基于LC-MS/MS技术在枸杞叶片中共检测到16个类型合计204个化合物,其中初生代谢产物以氨基酸类为主,次生代谢产物以有机酸类和黄酮类为主。枸杞瘿螨致瘿后可显著影响枸杞叶片代谢产物的含量,引起黄酮类、苯丙素类等30种代谢产物发生显著变化,其中有21种代谢物上调,9种代谢物下调,此外圣草酚、异鼠李素-3-O-新橙皮糖苷、东莨菪内酯等8种具有药理活性和生物活性的化合物含量显著上调。枸杞虫瘿叶片资源具有潜在的开发价值,值得深入研究。

Aceria pallida is one of the most common pests in the main production areas of Lycium barbarum in China.The mite mainly feeds on foliage,leading to local tissue deformation and formation of massive galls,which seriously affects the growth and yield of L.barbarum.However,little is known about the influence of galling organisms on plant primary and secondary metabolism.In order to compare the metabolites differences between healthy and the mite infested leaves of wolfberry,and provide a scientific basis for the development and utilization of the galled leaves,L.barbarum seedlings were infested with A.pallida artificially in the laboratory,the metabolites of L.barbarum leaves were determined by LC-MS/MS.Our results showed that the leaves were rich in amino acids and flavonoid compounds.A total of 204 compounds from 16 classes were detected in L.barbarum leaves based on LC-MS/MS.The primary metabolites are mainly amino acids,and the secondary metabolites are mainly organic acids and flavonoids.The content of the metabolite in the leaves of L.barbarum was significantly affected by the mite,30 metabolites such as flavonoids and phenylpropanoids were significantly changed,21 metabolites were up-regulated and 9 metabolites were down-regulated significantly.There were 8 compounds which has pharmacological and biological activity,such as eriodictyol,isorhamnetin-3-O-neohesperidoside and scopoletin up-regulated significantly.Based on the above findings,we suggest that the galled leaves of L.barbarum have a potential to be developed in the future.