人参三七川芎提取物对高糖高脂诱导血管衰老小鼠肠道菌群的影响

Effect of Ginseng Radix et Rhizoma,Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma extracts on intestinal flora of vascular aging mice induced by high glucose and high lipid

观察血管衰老小鼠肠道菌群的变化,探究血管衰老与肠道菌群的关系及人参三七川芎提取物对血管衰老小鼠肠道菌群的影响。以腹腔注射链脲佐菌素(STZ)联合喂养高脂饲料诱导小鼠血管衰老模型,生化法检测血清胆固醇(CHO)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)以及血糖(GLU),HE染色法检测小鼠胸主动脉形态,Western blot法检测小鼠胸主动脉周期蛋白依赖激酶抑制因子2A(p16)、周期依赖性蛋白激酶抑制因子1A(p21)蛋白表达。用Illumina MiSeq高通量测序技术检测小鼠肠道菌群16S rDNA基因,探索各组小鼠肠道菌群的变化。结果显示,中药低、高剂量组小鼠GLU水平降低,但各项血脂指标变化不明显,而二甲双胍能够明显下调小鼠GLU(P<0.01)、CHO和LDL-C水平(P<0.05),给药各组血管内膜损伤不明显,中膜少量增生,p16和p21蛋白表达明显下降(P<0.05)。各组小鼠肠道菌群在门水平上均由厚壁菌门(Firmicutes,F)和拟杆菌门(Bacteroidetes,B)组成为主,但是B/F比值有差异,与青年组和对照组相比,模型组B/F比值显著降低(P<0.01);与模型组相比,中药高剂量组和二甲双胍组B/F比值显著升高(P<0.05)。各组小鼠肠道中都存在优势菌群和差异菌群。结果表明,人参三七川芎提取物可以通过升高B/F比值,改善肠道菌群结构,营造良好的肠道环境,为其降低血糖血脂,延缓血管衰老提供新的可能通路。

The aim of this paper was to observe the changes of intestinal flora in vascular aging mice,in order to explore the relationship between vascular aging and intestinal flora and the effects of extracts of Ginseng Radix et Rhizoma,Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma on intestinal flora of vascular aging mice.A model of vascular aging in mice was induced through intrape-ritoneal injection with streptozotocin(STZ)combined with high-fat diet.Biochemical detection was performed on serum cholesterol(CHO),triglyceride(TG),high-density liptein cholesterol(HDL-C),low-density liptein cholesterol(LDL-C)and blood glucose(GLU).HE staining was used to detect mice thoracic aorta morphology,and the expressions of cyclin-dependent kinase inhibitor 2 A(p16)and cyclin-dependent kinase inhibitor 1 A(p21)protein in mice thoracic aorta were detected by Western blot.The 16 S rDNA gene of mice intestinal flora was detected by Illumina MiSeq high-throughput sequencing technology to explore the changes of intestinal flora in each group.The results demonstrated that the GLU level in low-dose and high-dose TCM groups decreased,but with unobvious changes in blood lipid indexes.Metformin could significantly decrease the levels of GLU(P<0.01),CHO and LDL-C in mice(P<0.05).Intravascular injury was not obvious in each drug group,and the expressions of p16 and p21 protein were significantly decreased(P<0.05).The intestinal flora of each group was mainly composed of Firmicutes(F)and Bacteroidetes(B)at the level of the phylum,but the B/F ratio was different from that of the youth group and the blank control group.The B/F ratio of the model group was significantly lower(P<0.01),and compared with the model group,the B/F ratio of the high-dose group and the metformin group was signi-ficantly higher(P<0.05).There were dominant and differential floras in the intestine of each group of mice.The results showed that extracts of Ginseng Radix et Rhizoma,Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma could improve the intestinal flora structure and create a good intestinal environment by increasing the B/F ratio,which provides a new possible pathway for lowering blood glucose and blood lipids and delaying vascular aging.