尾加压素Ⅱ促进毛乳头细胞增殖和迁移的实验研究

Effect of urotensinⅡon the proliferation and migration of dermal papilla cell

目的探讨尾加压素Ⅱ(UⅡ)对体外培养的毛乳头细胞增殖和迁移的影响。方法首先,选择10^-5 mol/L、10^-6 mol/L、10^-7 mol/L、10^-8 mol/L、10^-9 mol/L、10^-10 mol/L 6个浓度的UⅡ作用于体外培养的大鼠毛乳头细胞48 h,阴性对照组为使用不含UⅡ培养基培养的毛乳头细胞。采用CCK-8法检测毛乳头细胞的增殖活性。其次,采用Transwell实验检测10-6 mol/L和10-7 mol/L的UⅡ对毛乳头细胞体外迁移的影响。同时进行划痕实验,用一次性枪头在铺满90%培养皿底的毛乳头细胞上划痕后,加入含10-6 mol/L UⅡ的DMEM培养基,分别于0 h和24 h测量毛乳头细胞爬行的距离。结果与阴性对照组相比,UⅡ可促进毛乳头细胞增殖,其中10^-6 mol/L UⅡ组增殖率高达(41±4.4)%,差异有统计学意义(P<0.05);Transwell检测结果显示,10^-6 mol/L和10^-7 mol/L UⅡ处理组穿过Transwell滤膜的细胞数明显多于对照组,差异有统计学意义(P<0.05);划痕实验结果显示,阴性对照组及10^-6 mol/L UⅡ组24 h创面愈合指数分别为0.18±0.13、0.78±0.09,差异有统计学意义(P<0.05)。结论10^-6 mol/L、10^-7 mol/L和10^-8 mol/L UⅡ可促进大鼠毛乳头细胞增殖,10^-6 mol/L和10^-7 mol/L UⅡ可促进大鼠毛乳头细胞迁移。

Objective To investigate the effect of urotensinⅡ(UⅡ)on the proliferation and migration of dermal papilla cell.Methods First,the dermal papilla cells were cultured and treated with urotensinⅡof different concentrations(including 10^-5 mol/L,10^-6 mol/L,10^-7 mol/L,10^-8 mol/L,10^-9 mol/L,10^-10 mol/L)for 48 h.The negative control group was supplemented with culture medium without urotensinⅡ.Then the proliferation of cells was evaluated by CCK8 method.Second,the effect of 10^-6 mol/L and 10^-7 mol/L urotensinⅡon migration ability of dermal papilla cells was detected by Transwell experiment.At the same time,the dermal papilla cells were cultured in 12-well plates and a single scratch wound was made in each well.Then the cells were treated with 10^-6 mol/L urotensinⅡ.The distance of cell migration at 0 h and 24 h was measured respectively.Results Compared with the control group,UrotensinⅡcould promote the proliferation of dermal papilla cell,and the proliferation rate was as high as(41±4.4)%in the 10^-6 mol/L urotensinⅡtreatment group,with statistically significant difference(P<0.05).Transwell results showed that the number of cells passing through transwell membrane in the 10^-6 mol/L and 10^-7 mol/L urotensinⅡtreatment group was significantly higher than that in the control group(P<0.05).The results of wound healing assay showed that the wound healing indexes of the control group and the 10^-6 mol/L urotensinⅡtreatment group at 24 h were 0.18±0.13 and 0.78±0.09,respectively,with statistically significant difference(P<0.05).Conclusion 10^-6 mol/L,10^-7 mol/L,and 10^-8 mol/L urotensinⅡcould promote the proliferation of dermal papilla cells,and 10-6 mol/L and 10^-7 mol/L urotensinⅡcan promote the migration of dermal papilla cell.