电针对高血脂合并脑缺血大鼠海马齿状回星形胶质细胞活化的影响

Effect of electroacupuncture on GFAP in hippocampal dentate gyrus(DG) of rats with hyperlipidemia and middle cerebral ischemia

目的观察电针对高血脂合并脑缺血大鼠海马齿状回星形胶质细胞活化的影响。方法 SD成年雄性大鼠随机分为正常组、模型组、假手术组、电针1组和电针2组。高血脂阶段:饲喂高脂饲料42 d造模,电针1组电针丰隆穴,每天1次,共7 d,电针2组大鼠不做任何干预。合并脑缺血阶段:行Fecl3诱导的脑缺血模型手术。术后电针组均电针丰隆及百会穴,每天1次,共14 d。手术后第1天对所有大鼠进行神经功能缺损评分,HE染色观察神经元细胞形态学变化。实验完成后检测血清中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)水平,免疫组化法检测缺血侧大脑海马齿状回和皮层缺血半暗带胶质纤维酸性蛋白(GFAP)的表达增加。结果与正常组比较,模型组大鼠TC、TG、LDL升高(P<0.05),电针组大鼠TG、LDL降低(P<0.01);模型组大鼠神经功能缺损评分增高,HE染色可见明显核固缩,组织排列散乱,海马齿状回和皮层缺血半暗带GFAP表达增加。与模型组相比,电针组神经功能缺损评分降低。HE染色可见神经元细胞形态有所恢复,海马齿状回和皮层半暗带GFAP表达在术后第1天减少,第7天增加,第14天减少。结论高血脂阶段电针干预配合脑缺血后电针干预,可降低血脂水平改善大鼠神经功能,调节大鼠海马星形胶质细胞的活化,从而促进脑损伤神经细胞的修复。

Objective To observe the effect of electroacupuncture on GFAP in hippocampal dentate gyrus(DG) of rats with hyperlipidemia and middle cerebral artery thrombus. Methods Adult male SD rats were randomly divided into normal group, model group, sham-operation group, electroacupuncture 1(EA1) group and electroacupuncture 2(EA2) group. Hyperlipidemia stage: Rats in model and EA group were fed with high-fat diet for 42 days, while rats in EA1 group were needled at ST40 once a day for 7 days. Cerebral ischemia stage: Rats in model group and EA groups were induced by 50% FeCl3 to establish hyperlipidemia with cerebral ischemia. After operation, the EA groups needled at ST40 and GV20 once a day for 14 days. All rats were scored for neurological function. The levels of total cholesterol(TC), triglyceride(TG), high density lipoprotein(HDL) and low-density lipoprotein(LDL) in serum were detected on the 1 st, 7 th and 14 th days. The morphological changes of neuron cells were observed by using Hematoxylin-eosin(HE) staining. Immunohistochemical method was used to detect the expression of glial fibrillary acidic protein(GFAP) in DG and ischemic cerebral region. Results Compared with normal group, TC, TG and LDL in the model group increased(P<0.05), TG and LDL in the EA group decreased(P<0.01). In the model group, the neurological function score(NDS) was increased. HE staining showed obvious nuclear consolidation and contraction, scattered tissue arrangement. The expression of GFAP increased in the DG and ischemic cerebral regions(P<0.01). In EA group, NDS decreased. HE staining showed the appearance of neurons was improved. GFAP expressions in the DG and ischemic cerabral regions decreased at day 1, increased at day 7 and decreased again at day 14 after operation. Conclusion EA intervention in hyperlipidemia stage can reduce blood lipid level, improve neurological function, regulate activation of astrocytes and promote neurorepair after hyperlipidemia-ischemia injury.