CXCL12调节骨肉瘤MG-63细胞的增殖、侵袭及机制研究

CXCL12 regulates the proliferation,invasiveness of human osteosarcoma MG-63 cells

目的研究趋化因子CXCL12对人骨肉瘤MG-63细胞增殖、侵袭的影响。方法在体外用不同浓度CXCL12处理MG-63细胞后,应用MTT方法、Transwell侵袭实验、western blotting方法,检测CXCL12对MG-63细胞增殖、侵袭及金属基质蛋白酶MMP-9表达的影响。结果CXCL12作用后,人骨肉瘤MG-63细胞增殖、侵袭能力明显增强;且CXCL12增加基质金属蛋白酶MMP-9的表达水平。结论CXCL12在体外可促进人骨肉瘤MG-63细胞的增殖,可通过调节MMP-9的表达水平进而调节骨肉瘤细胞的侵袭。

Objective To investigate the effects of CXCL12 on the proliferation,invasiveness on human osteosarcoma MG-63 cells.Methods MG-63 cells were treated with recombinant human CXCL12,the effects of CXCL12 on proliferation,invasion and matrix metalloproteinase-9(MMP-9)expression were detected by MTT assay,transwell invasion and western blotting respectively.Results The proliferation,invasiveness of MG-63 cells significantly enhanced and the expression of MMP-9 increased after CXCL12 treatment.Conclusion CXCL12 promotes the proliferation of MG-63 cells and regulates the invasiveness of MG-63 cells through mediating the expression of MMP-9.