摘要
3-甾酮-Δ~1-脱氢酶是甾体化合物微生物代谢的关键酶,负责催化3-酮基类甾体化合物A环上的C1,2位脱氢反应。其不仅在甾体母核的早期降解途径中发挥重要作用,而且能通过A环C1,2位上双键的导入显著提高甾体化合物的生理活性。本文详细阐述了3-甾酮-Δ~1-脱氢酶在微生物中的种属分布和序列特征、酶的生物学特性、生理作用、催化机理以及分子改造等,为深入研究该酶在甾体生物转化领域中的应用提供重要参考。
3-ketosteroid-Δ~1-dehydrogenase is one of the key enzymes in microbial steroid catabolism,which catalyzesΔ~1-dehydrogenation of A ring of 3-ketosteroid substrates.The enzyme not only plays a critical role in the early step of the degradation of the steroid nucleus,but also introduces the double bond at the C1,2 position of the A ring to significantly improve the physiological activity of the steroidal compound.In this paper,the species distribution in microorganisms and sequence characteristics,biological properties,physiological role,catalytic mechanism and molecular modification of 3-ketosteroid-Δ~1-dehydrogenase are reviewed.It provides reference for further study of the 3-ketosteroid-Δ1-dehydrogenase application in the steroid biotransformation field.
作者
贾红晨
李芳
郑鑫铃
崔慧林
骆健美
申雁冰
王敏
田妥
丁安鹏
JIA Hong-Chen;LI Fang;ZHENG Xin-Ling;CUI Hui-Lin;LUO Jian-Mei;SHEN Yan-Bing;WANG Min;TIAN Tuo;DING An-Peng(Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,Tianjin Key Laboratory of Industrial Microbiology,Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control,College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China;Key Laboratory of Molecular Microbiology and Technology of Ministry of Education,Nankai University,Tianjin 300457,China;Tianjin Tianyao Pharmaceuticals Company Limited,Tianjin 300462,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2020年第7期2218-2235,共18页
Microbiology China
基金
国家重点研发计划(2019YFA0905300)
国家自然科学基金(21978220)
天津市自然科学基金(18JCZDJC 32500)
天津市企业科技特派员项目(19JCTPJC50800)
南开大学分子微生物学与技术教育部重点实验室开放课题
天津市大学生创新创业训练计划(201910057099)。
关键词
3-甾酮-Δ~1-脱氢酶
甾体C1
2脱氢反应
生物学特性
生理作用
催化机理
3-ketosteroid-Δ~1-dehydrogenase
Δ~1-dehydrogenation of steroid
Biological characteristics
Physiological role
Catalytic mechanism
