放射治疗对肺癌患者外周血中DC细胞的影响

The Effect of Radiotherapy on DC Cell in Peripheral Blood of Patients with Lung Cancer

目的:研究接受放射治疗的非小细胞肺癌患者放疗前及放疗后DC细胞的变化.方法:对入院治疗的30例非小细胞肺癌放疗患者分别在放疗前1周、放疗2000cGy、4000cGy、6000cGy通过免疫磁珠法检测DC细胞数、存活DC细胞数、凋亡DC细胞数和DC细胞存活率的检测.结果:DC细胞计数于放疗2000cGy、4000cGy、6000cGy逐渐升高,F=8.53,P<0.001;接受一定剂量放射治疗后DC细胞存活数呈在开始一段剂量升高而后逐渐降低,放疗4000cGy时最高,放疗6000cGy时下降,放疗4000cGy与放疗2000cGy、放疗6000cGy比较F=6.88,P<0.05;DC细胞凋亡数的变化无明显规律,放疗4000cGy时最高,放疗6000cGy与放疗前1周、放疗2000cGy、放疗4000cGy比较F=3.68,P=0.015;DC细胞存活率放疗前与放疗后的变化无统计学差异,F=0.839,P=0.476.结论:非小细胞肺癌患者中的DC细胞水平随放疗剂量的变化而发生变化,且放射治疗可提高体内DC细胞的抗原提呈作用.

Objective:To discuss the dynamic changes of DC cell in peripherd blood of patients with lung cancer before and after different radiotherapy dose.Methods:30 patients with lung cancer were included in the study.To detect DC cell count,live DC cell number,DC number of apoptotic cells and DC cell activity in Peripheral blood of 30 lung cancer radiotherapy patients by MACS(magnetic activated cell sorting)before and after radiotherapy.Results:The number of DC cells increased gradually at 2000cGy,4000cGy and 6000cGy by radiotherapy,F=8.53,P<0.001.The number of live DC cells increased at first and then decreased.The highest level at 4000cGy by radiotherapy,and declined at 6000cGy,and 4000cGy by radiotherapy,F=6.88 and P<0.05 compared with 2000cGy and 6000cGy by radiotherapy.There was no significant change in the number of apoptotic cells in DC,which was the highest at 4000cGy by radiotherapy.The dose of 6000cGy compared with 1 week before radiothrapy,the dose of 2000cGy and 4000cGy,F=3.68 and P=0.015.The changes of the activity of DC cells in the serum of the patients with lung cancer before and after radiotherapy have no significant difference in the activity of DC cells,F=0.839,P=0.476.Conclusion:Radiation therapy can increase the level of DC cells in peripheral blood of lung cancer patients and promote the action of tumor antigen.