培养瓶制备腺病毒的收率研究

Improvement of the process for adenovirus production

在293细胞培养体系中感染腺病毒后,分别培养48、72或96 h时收获病毒,比较产量和活性,结果表明培养72 h是高产的最佳收获时机;并讨论了毒种制备的规模、后续使用特点对制备方法的限制。

After infection with adenovirus in 293 cell culture system, adenovirus was harvested from 48 h, 72 h and 96 h culture, respectively. The yield and activity of the adenovirus were compared, and the optimum harvest time for high yield adenovirus was judged to be 72 h. The limitations of the scale of adenovirus preparation and the characteristics of subsequent use on the preparation were discussed.