抗癌Ⅰ号方含药血清对HepG 2肝癌细胞增殖抑制的影响

The Effect of Anticancer Formula I on the Proliferation Inhibition of HepG2 Hepatoma Cells

目的研究抗癌Ⅰ号方含药血清对HepG2肝癌细胞的抑制作用及其可能机制。方法用MTT法检测不同浓度的抗癌Ⅰ号方含药血清对HepG2肝癌细胞的增殖抑制作用。免疫印迹法检测各浓度组48h PI3K/Akt信号通路重要蛋白Akt及其磷酸化形式p-Akt的蛋白水平。结果不同浓度的抗癌Ⅰ号方含药血清对HepG2肝癌细胞均有抑制作用,且有一定的浓度依赖性。随着浓度升高,p-Akt/Akt值呈降低趋势,差异有统计学意义(P <0.05)。结论抗癌Ⅰ号方含药血清对HepG2肝癌细胞增殖起抑制作用,同时与药物浓度有一定的依赖性,其可能通过抑制PI3K/Akt通路激活实现。

Objective To study the inhibitory effect of anticancer Ⅰ on HepG2 hepatoma cells and its mechanism. Methods MTT method was used to detect the inhibitory effect of anticancer Ⅰ on HepG2 hepatoma cells. Western blotting was used to detect the protein levels of the important protein Akt and phosphorylated p-Akt of PI3K/Akt signaling pathway in each concentration group at 48 h. Results Different concentrations of anti-cancer Ⅰ have inhibitory effect on HepG2 hepatoma cells, and there is certain concentration dependence. With the increase of the concentration, the p-Akt/Akt value decreased, and the difference was statistically significant(P<0.05). Conclusion Anti cancer formula Ⅰ can inhibit the proliferation of HepG2 hepatoma cells, and it is dependent on the drug concentration, which may be achieved by inhibiting the activation of PI3K/Akt pathway.