蓝莓组培苗在几种新型间歇浸没生物反应器中增殖培养效果的比较

Comparison on Multiplication of Vaccinium corymbosum Plantlets in Several New Type Temporary Immersion Bioreactors System

以蓝莓第3代健壮无根组培苗为试材,采用3种不同生物反应器(摇摆式、气雾式和双瓶式)进行增殖培养,对蓝莓组培苗的生理生化指标进行了比较分析,以期选择出最佳的增殖培养生物反应器和培养方法。结果表明:新型生物反应器极显著提高了增殖系数,是对照的2.0~2.5倍,增殖效率综合分析,气雾式优于摇摆式优于双瓶式;新型生物反应器培养的增殖苗各项生长指标均极显著优于对照,生长指标综合最优的是双瓶式培养;新型生物反应器能显著降低增殖苗的褐化程度。

The third generation of blueberry robust rootless tissue culture plantlets were used as the test materials.The multiplication coefficient,growth indexes and physiological indexes of the proliferation plantlets in different bioreactors(swing type,aerosol type,double bottle type)were studied,so as to select the best proliferation culture bioreactor and culture method.The results showed that the multiplication coefficient had been significantly improved in the new bioreactors,and it was 2.0-2.5 times to the control.Comprehensive analysis of multiplication efficiency,the aerosol type better than the swing type better than the double bottle type.The growth indexes of the multiplication seedlings in the new bioreactors were significantly better than those of the control,and the optimal growth indexes was double bottle culture,and the new bioreactor could significantly lower the browning degree of the multiplication seedlings.