摘要
以广藿香组培苗和紫苏苗为试材,采用室内盆栽的方法,分别设定3个不同种植方式处理(连作组(第2茬广藿香单作)、间作组(第2茬广藿香与紫苏间作)和对照组(第1茬广藿香单作)),通过测定广藿香农艺性状、叶片丙二醛(MDA)含量、根际土壤微生物数量与土壤酶活性、药材中百秋李醇和广藿香酮含量,研究间作紫苏的种植方式对广藿香连作障碍的缓解效应。结果表明:培育90 d后,广藿香农艺性状(株高、干质量、鲜质量、主根长)及百秋李醇和广藿香酮的含量,间作组显著高于连作组(P<0.05),而与对照组无显著性差异。叶片MDA含量,间作组较连作组减少了33.74%(P<0.05),而与对照组无显著性差异。土壤细菌和放线菌数量,间作组较连作组分别增加了30.35%、66.67%(P<0.05),间作组较对照组分别增加了21.24%、75.00%(P<0.05)。土壤真菌数量,间作组与对照组无显著性差异。土壤蔗糖酶、脲酶和酸性磷酸酶活性,间作组较连作组分别升高了25.50%、30.58%和50.22%(P<0.05),而土壤蔗糖酶活性,间作组与对照组无显著性差异,脲酶和酸性磷酸酶活性,间作组较对照组分别升高了7.44%、41.92%(P<0.05)。综上所述,广藿香间作紫苏可在一定程度上缓解广藿香连作障碍。
Taking Pogostemon cablintissue culture seedlings and Perilla frutescens seedlings as materials and adopting a method of the pot culture in house,there were 3 treatments,continuous cropping group(only the second crop Pogostemon cablin),intercropping group(the second crop Pogostemon cablin intercropping with perilla frutescens),control group(only the first crop Pogostemon cablin),and the agronomic traits,the content of malondialdehyde(MDA)in leaves,microbial quantities and soil enzyme activities in rhizosphere soil,the content of patchouli alcohol and pogostone in Pogostemon cablin were determined,in order to study the relieving effect of Pogostemon cablinintercropping with Perilla frutescens on its continuous cropping obstacle.The results showed that after 90 days of cultivation,the agronomic traits(plant height,dry weight,fresh weight,main root length)and the content of patchouli alcohol and pogostone were significantly higher in the intercropping group than in the continuous cropping group(P<0.05),and there were no significant differences between intercropping group and control group.For the content of MDA in the leaves,intercropping group was reduced by 33.74%(P<0.05)compared with the continuous cropping group,but there was no significant difference between intercropping group and control group.Compared with the continuous cropping group,the number of soil bacteria and actinomycetes in Intercropping group increased by 30.35%and 66.67%(P<0.05),respectively,and the intercropping group increased by 21.24%and 75.00%(P<0.05),respectively,compared with the control group.There was no significant difference in the number of soil fungi between the intercropping group and the control group.The activities of soil invertase,urease and acid phosphatase in intercropping group were increased by 25.50%,30.58%and 50.22%(P<0.05),respectively than the continuous cropping group.The soil invertase activity was not significant difference between the intercropping group and the control group.While urease and acid phosphatase activities in intercropping group were increased by 7.44%and 41.92%(P<0.05),respectively,compared with the control group.It was concluded that the intercropping of Perilla frutescens could alleviate the continuous cropping obstacle in Pogostemon cablinto a certain extent.
作者
周界
潘丽萍
李明
ZHOU Jie;PAN Liping;LI Ming(School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou,Guangdong 510006)
出处
《北方园艺》
CAS
北大核心
2020年第13期111-117,共7页
Northern Horticulture
基金
国家重点研发计划资助项目(2017YFC1700704)
广东省科技计划资助项目(2013B020503066,2014A020208135,2016A020226050)。
关键词
广藿香
紫苏
间作
连作障碍
Pogostemon cablin
Perilla frutescens
intercropping
continuous cropping obstacle