核转录因子κB、Toll样受体和炎性细胞因子在颅内动脉瘤破裂中的表达及相关性

Expression and correlation of nuclear factor kappa B,Toll-like receptor and inflammatory cytokines in rupture of intracranial aneurysms

目的探究核转录因子κB(NF-κB)、Toll样受体(TLR)和炎性细胞因子在颅内动脉瘤(IA)破裂中的表达及相关性。方法通过手术收集IA以及IA破裂的动脉瘤组织作为IA组和IA破裂组,同时收集健康血管组织作为对照组。通过qPCR检测动脉瘤组织中NF-κB、TLR4、白细胞介素1β(IL-1β)以及超敏C反应蛋白(hs-CRP)mRNA水平,通过Western blot检测NF-κB和TLR4蛋白水平。酶联免疫吸附法检测各组血清中IL-1β和hs-CRP的水平。结果IA组和IA破裂组动脉瘤组织中的NF-κB、TLR4、IL-1β、hs-CRP mRNA和NF-κB、TLR4蛋白水平显著高于对照组(P<0.05),此外,IA破裂组动脉瘤组织中的NF-κB、TLR4、IL-1β、hs-CRP mRNA和NF-κB、TLR4蛋白水平显著高于IA组(P<0.05)。IA组和IA破裂组血清IL-1β和hs-CRP显著高于对照组(P<0.05),IA破裂组血清IL-1β和hs-CRP水平显著高于IA组(P<0.05)。结论与IA患者相比,IA破裂患者的动脉瘤组织中具有更高水平的NF-κB、TLR4、IL-1β以及hs-CRP mRNA和蛋白的表达,并且血清中IL-1β和hs-CRP的水平也更高,这说明NF-κB、TLR4、IL-1β以及hs-CRP可能与IA破裂有关。

Aim To investigate the expression and correlation of nuclear factorκB(NF-κB),Toll-like receptors(TLR)and inflammatory cytokines in the rupture of intracranial aneurysm(IA).Methods The IA and IA ruptured aneurysm tissues were collected by surgery as the IA group and the IA rupture group,and healthy vascular tissues were collected as a control group.The levels of NF-κB,TLR4,interleukin 1β(IL-1β)and hypersensitive C-reactive protein(hs-CRP)mRNA in aneurysm tissues were detected by qPCR.NF-κB and TLR4 protein levels were detected by Western blot.The levels of IL-1βand hs-CRP in the serum of each group were detected by enzyme-linked immunosorbent assay.Results The levels of NF-κB,TLR4,IL-1βand hs-CRP mRNA and protein in the aneurysm of IA group and IA rupture group were significantly higher than those of the control group(P<0.05).The levels of NF-κB,TLR4,IL-1βand hsCRP mRNA and protein in the aneurysm of IA rupture group were significantly higher than those of IA group(P<0.05).Serum IL-1βand hs-CRP were significantly higher in the IA group and the IA rupture group than in the control group(P<0.05).The levels of serum IL-1βand hs-CRP in the IA rupture group were significantly higher than those in the IA group(P<0.05).Conclusion Compared with IA patients,IA-ruptured patients have higher levels of NF-κB,TLR4,IL-1β,and hs-CRP mRNA and protein expression in aneurysms,suggesting that NF-κB,TLR4,IL-1β,and hsCRP are involved in IA rupture.