摘要
目的:探究艾灸足三里对自然衰老大鼠皮肤衰老的影响。方法:选取自然衰老大鼠连续足三里艾灸6个月,H&E和马松染色观察皮肤组织形态和纤维化,检测氧化指标SOD活力和MDA含量,qPCR检测皮肤中多种生长因子的表达,ELISA检测皮肤中雌二醇的含量,Western Blot检测皮肤胶原蛋白的变化。结果:与对照组比较,艾灸组能明显增加皮肤中多种生长因子的表达,增加胶原蛋白和雌二醇的含量,减少衰老带来的皮肤氧化。结论:艾灸能显著缓解老龄鼠的皮肤衰老。
Objective:To investigate the effects of moxibustion at“Zusanli”on skin aging in senile rats.Methods:SD rats accepted consecutive moxibustion for 6 months.The skin tissues were observed by H&E.Superoxide dismutase(SOD)activity and malondialdehyde(MDA)content was determined by the instructions.The expression of various growth factors in the skin was detected by qPCR.The content of estradiol in the skin was detected by ELISA.The expression of collagen in skin were detected by Western Blot.Results:Compared with the control group,moxibustion group can significantly increase the expression of various growth factors in the skin,increase the content of collagen and estradiol,and reduce the skin oxidation caused by aging.Conclusion:Moxibustion can significantly alleviate the aging of old mice skin.
作者
张苗
纪峰
陈友义
ZHANG Miao;JI Feng;CHEN You-yi(Fujian University of Traditional Chinese Medicine,Fuzhou 350112,China)
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2020年第7期912-916,共5页
JOURNAL OF BASIC CHINESE MEDICINE
基金
福建省教育厅中青年教师科研项目(JAT160246)-逆灸足三里对自然更年期大鼠皮肤E2、VEGF及EGFmRNA表达的影响
福建省卫计委青年科研项目(2016-2-24)-逆灸足三里对自然更年期大鼠皮肤雌激素受体β(ERβ)及TGF-β1、b-FGFmRNA表达的影响。
关键词
艾灸
足三里
衰老皮肤
Moxibustion
Zusanli
Aging skin