宫颈病变中miR-34a、miR-199a表达及与高危型HPV感染关系

The expressions of miR-34a and miR-199a in cervical lesions tissue and their correlations with high risk HPV infection

目的:探讨宫颈病变中微小RNA-34a(miR-34a)、微小RNA-199a(miR-199a)的表达及与高危型人乳头瘤病毒(HPV)感染关系。方法:选择2016年3月-2018年11月本院宫颈病变筛查的306例女性,行薄层液基细胞学检查(TCT)、HPV基因分型检测,对TCT阳性者行阴道镜组织病理学活检,根据HPV基因分型检测分为高危型HPV阳性组和阴性组。采用SYBR Green荧光定量PCR法检测宫颈脱落细胞miR-34a、miR-199a表达,比较各组高危型HPV感染及miR-34a、miR-199a表达。结果:病理结果显示宫颈癌45例(宫颈癌组),宫颈上皮内瘤变(CIN)111例(CINⅠ42例、CINⅡ33例、CINⅢ36例),正常宫颈/慢性炎症150例(对照组)。检出高危型HPV感染87例,阳性率在对照组、CINⅠ级组、CINⅡ级组、CINⅢ级组、宫颈癌组依次升高(P<0.05);高危型HPV阳性感染中HPV16感染检出率最高,其次为HPV18。宫颈脱落细胞miR-34a、miR-199a表达在对照组、CINⅠ级组、CINⅡ级组、CINⅢ级组、宫颈癌组依次降低(P<0.05),高危型HPV阳性组miR-34a、miR-199a表达均低于阴性组(P<0.05);Pearson相关分析显示,宫颈病变者宫颈脱落细胞miR-34a、miR-199a表达与CIN分级、高危型HPV感染均呈负相关(P<0.05)。结论:随着宫颈病变加重,宫颈脱落细胞miR-34a、miR-199a表达降低,高危型HPV感染率增高,两者呈负相关;其中HPV16感染检出率最高。miR-34a、miR-199a可能成为宫颈癌早期筛查的有效指标。

Objective:To investigate the expressions of microRNA-34a(miR-34a)and microRNA-199a(miR-199a)in cervical lesions tissue,and to study their relationships with high-risk human papillomavirus(HPV)infection.Methods:From March 2016 to November 2018,306 women who were screened for cervical lesions were selected as the study objects,and they all were examined by thin layer liquid based cytology(TCT)and HPV-DNA.Among them,the women with TCT positive were given colposcopy histopathological biopsy,and were divided into high-risk HPV positive group and negative group according to the result of HPV-DNA test.The expression of miR-34a and miR-199a in cervical abscission cells was detected by SYBR Green fluorescence quantitative PCR,and the expression of miR-34a and miR-199a,and the rate of high-risk HPV infection were compared among these groups.Results:Pathological results showed that 45 women with cervical cancer(in group A),111 women with cervical intraepithelial neoplasia(CIN)(42 women with CINⅠin group B1,33 women with CINⅡin group B2,and 36 women with CINⅢin group B3),and 150 women with normal cervix or cervical chronic inflammation(in group C).There were 87 women with high-risk type HPV infection,and the positive rate of HPV infection of women in group C,group B1,group B2,group B3,and group A had increased in turn(P<0.05).The detection rate of HPV16 infection was the highest in the women with high-risk HPV positive infection,followed by HPV18.the expressions of miR-34a and miR-199a in cervical exfoliated cells of women in group C,group B1,group B2,group B3,and group A had decreased in turn,and the expressions of miR-34a and miR-199a of women with high-risk HPV positive infection were significant lower than those of women without HPV infection(P<0.05).Pearson correlation analysis showed that the expressions of miR-34a and miR-199a in cervical exfoliated cells of women with cervical lesions were negatively correlated with CIN classification and high-risk HPV infection(P<0.05).Conclusion:With the aggravation of cervical lesions,the expressions of miR-34a and miR-199a in cervical exfoliated cells decrease and the infection rate of high-risk HPV increase,the expressions of miR-34a and miR-199a are negatively correlated with the infection rate of high-risk HPV.The detection rate of HPV16 infection is the highest.The expressions of miR-34a and miR-199a can be effective indicator for early screening of cervical cancer.