FAT1在结直肠癌中的表达及临床意义

Expression and Clinical Significance of FAT1 in Colorectal Cancer

目的探讨脂肪非典型钙黏蛋白1(FAT1)在结直肠癌中的表达及其对肠癌细胞株侵袭和迁移能力的影响。方法选取该院2010年1月—2014年12月诊治的结直肠癌患者85例,直肠癌组织及正常肠黏膜组织内FAT1表达水平选取免疫组化染色技术与RT-PCR进行检测,构建FAT1过表达和RNA干扰载体,分别转染SW620细胞株,肠癌细胞迁移及侵袭能力改变情况选取Transwell小室检测。结果3种组织内FAT1蛋白均有表达,且表达强度呈递增趋势,其中正常黏膜组织阴性14.1%、低表达80.00%、中表达5.9%、高表达0.0%;结肠癌组织阴性0.0%、低表达21.2%、中表达36.5%、高表达42.4%;淋巴结转移癌组织阴性0.0%、低表达5.7%、中表达34.3%、高表达60.0%。FAT1表达状态与淋巴结转移相关(P<0.05)。体外细胞实验显示,上调FAT1表达可促进SW620细胞迁移和侵袭。结论FAT1可能是结直肠癌中潜在的促癌基因,其表达上调与结直肠癌的恶性演进密切相关。

Objective To investigate the expression of fat atypical cadherin 1(FAT1)in colorectal cancer and its effect on the invasion and migration of intestinal cancer cell lines.Methods 85 patients with colorectal cancer diagnosed and treated in the hospital from January 2010 to December 2014 were selected.The expression level of FAT1 in rectal cancer tissues and normal intestinal mucosa tissues was selected for detection by immunohistochemical staining and RT-PCR.The expression and RNA interference vectors were transfected into SW620 cell line,respectively,and the changes of intestinal cancer cell migration and invasion ability were selected for detection by Transwell cell.Results FAT1 protein was expressed in all three tissues,and the expression intensity showed an increasing trend,including normal mucosal tissue negative 14.1%,low expression 80.00%,medium expression 5.9%,high expression 0.0%;colon cancer tissue negative 0.0%,low expression 21.2%,medium expression 36.5%,high expression 42.4%;lymph node metastasis tissue negative 0.0%,low expression 5.7%,medium expression 34.3%,high expression 60.0%.The expression status of FAT1 was correlated with lymph node metastasis(P<0.05).In vitro cell experiments show that up-regulating FAT1 expression can promote the migration and invasion of SW620 cells.Conclusion FAT1 may be a potential oncogene in colorectal cancer,and its up-regulation is closely related to the malignant progression of colorectal cancer.