钙粘蛋白11在头颈部鳞癌中相关作用的研究

Study on the Relative Role of CDH11 in Head and Neck Squamous Cell Carcinoma

目的:研究钙粘蛋白11(cadherin 11,CDH11)在头颈部鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)细胞中的表达情况,以及CDH11对HNSCC细胞功能的影响。方法:应用Western印迹法(Western blot)检测在HNSCC细胞系及正常细胞中CDH11的表达差异,检测应用转染技术降低CDH11表达后,HNSCC细胞中上皮间充质转化标志物表达水平的变化情况,并对转染后的细胞形态、增殖能力及侵袭能力进行测定。结果:HNSCC细胞中的CDH11及E-钙粘蛋白(E-cadherin)表达高于正常细胞,Twist家族转录因子-1(Twist family BHLH transcription factor 1,Twist-1)表达低于正常细胞,提示高水平CDH11可能抑制上皮间充质转化过程。转染后,HNSCC细胞中E-钙粘蛋白表达降低,Twist-1表达升高,且细胞间粘附降低;细胞增殖能力较转染前均增强(P<0.05);细胞侵袭能力较转染前有所提高(P<0.05)。结论:CDH11通过抑制HNSCC细胞的上皮间充质转化过程,进而抑制了HNSCC的增殖和侵袭行为,提示CDH11在HNSCC中可能作为抑癌基因起作用。

Objective:To investigate the expression of cadherin11(CDH11)in head and neck squamous cell carcinoma(HNSCC)cells and the effect of CDH11 on the function of HNSCC cells.Methods:Western blot assay was used to detect the difference in the expression of CDH11 between HNSCC cell lines and normal cells.The changes in the expression levels of epithelial mesenchymal transformation markers in HNSCC cell lines was detected after transfection technology was used to reduce CDH11 expression.And the cell morphology,proliferation ability and invasion ability after transfection were measured.Results:The expression of CDH11 and E-cadherin in HNSCC cells was higher than that in normal cells,while the expression of Twist family BHLH transcription factor 1(Twist-1)was lower than that in normal cells.The high level of CDH11 may inhibit epithelial mesenchymal transformation.After transfection,E-cadherin expression in HNSCC cells was decreased,Twist-1 expression was increased,and intercellular adhesion was cut down.The proliferation capacity of the cells was enhanced compared with that before transfection(P<0.05).The invasion ability of cells was improved compared with that before transfection(P<0.05).Conclusion:CDH11 inhibits the epithelial mesenchymal transformation process of HNSCC cells,thereby inhibits the proliferation and invasion behavior of HNSCC,indicating that CDH11 plays an important role as a tumor suppressor gene in HNSCC.