基于艾里光片照明的随机光学重构显微系统的研制

Stochastic Optical Reconstruction Microscopy Based on Airy Light Sheet Illumination

基于单分子定位的随机光学重构显微镜(STORM)是观察亚细胞结构的重要工具,但是在实际应用中往往会受到多方面的限制。光片照明显微镜因为具有快速、高对比度、光损伤低等优点,在近些年也得到了快速的发展。但是传统的高斯光片照明因为会快速的发散而使成像的视野受到了限制,而艾里光束作为一种无衍射光束,表现出良好的传播不变性,应用在光片照明显微镜中可以获得更大的视野。研制了一套基于艾里光片照明的随机光学重构显微系统,并在100×100μm视野范围内实现对Thy1-YFP小鼠脑片进行连续光学切面的二维超分辨成像。

Stochastic optical reconstruction microscopy(STORM)based on single molecule localization is an important tool for observing subcellular structures,but in practical applications,it is often subject to restrictions.The rapid divergence of traditional Gaussian light-sheet limits the field-of-view(FOV).Airy beam,as a non-diffraction beam,which shows good propagation-invariance,can acquire larger field-of-view when used in light-sheet illumination microscopy.In this paper,a system combined stochastic optical reconstruction microscopy with Airy light-sheet illumination is developed,and two-dimensional super-resolution imaging of the continuous optical sections of Thy1-YFP mouse brain slice is realized within 100μm×100μm field-of-view.