血浆微小核糖核酸-155表达与冠状动脉慢血流的相关性研究

Study on correlation between expression of microRNA-155 in plasma and phenomenon of slow coronary flow

目的:探讨血浆微小核糖核酸-155(miR-155)表达水平与冠状动脉慢血流(CSF)现象的关系。方法:连续性选择2017-01—2019-06行冠状动脉造影检查符合CSF诊断标准的患者62例作为病例组,另随机选择同期接受冠状动脉造影检查无明显血管狭窄且冠脉血流速度正常的患者60例为对照组。采用校正的心肌梗死溶栓血流帧数(cTFC)法检测并计算平均心肌梗死溶栓治疗(TIMI)帧数值(mTFC),采用实时荧光定量PCR(RT-qPCR)法检测血浆miR-155表达水平。结果:病例组血浆miR-155相对表达水平和mTFC均显著高于对照组,差异有统计学意义(Z=3.219,P=0.001;Z=3.219,P=0.001)。Pearson相关性分析结果示:血浆miR-155水平与mTFC值呈正相关关系(r=0.322,P=0.000)。多因素Logistic回归分析结果示:血浆miR-155相对表达水平是CSF现象的独立危险因素(OR=1.597,95%CI 1.109~2.301,P=0.005)。ROC曲线分析结果示:血浆miR-155预测CSF的曲线下面积为0.669(0.574,0.764),cut-off值为24.19时,对应的灵敏度和特异度分别为53.2%和75.0%。结论:血浆miR-155表达水平与CSF现象密切相关,miR-155可作为CSF现象的早期预测因子。

Objective:To investigate the relationship between miR-155 in plasma and coronary slow flow(CSF)phenomenon. Method:A total of 62 CSF patients and 60 controls who underwent coronary angiography(CAG)in our hospital from January 2017 to June 2019 were selected into this study.Correct thrombolysis in myocardial infarction frame count(cTFC)was used to calculate the mean thrombolysis in myocardial infarction flame counts(mTFC).Real-time quantitative PCR(RT-qPCR)was used to determine the expression of miR-155 in plasma. Result:The plasma miR-155 level and mTFC in case group were both significantly higher than those in control group(Z=3.219,P=0.001;Z=3.219,P=0.001).Pearson correlation analysis showed that there was a positive correlation between plasma miR-155 and mTFC(r=0.322,P=0.000).Multiple Logistics regression analysis showed that plasma miR-155 was independent risk factor for CSF(OR=1.597,95%CI 1.109-2.301,P=0.005).The area under the ROC curve of miR-155 predicting for CSF was 0.669(0.574,0.764).When the cut-off value was set at 24.19,the Youden index reached the maximum with a sensitivity of 0.532 and a specificity of 0.750.Conclusion:The expression level of miR-155 in plasma is significantly correlated with CSF,and miR-155 could be used as a predictor of CSF.