西安地区RhD阴性患者血清学表型和不规则抗体调查

Investigation of serological phenotype and irregular antibodies in RhD-negative patients in Xi’an Area

目的:统计西安地区初筛RhD阴性住院患者Rh表型分布特点及不规则抗体检出情况,研究不同表型与抗-D抗体产生的相关性。方法:对633例初筛RhD阴性住院患者样本用Rh血型分型卡进行Rh血清学表型鉴定,用间接抗人球蛋白微柱凝胶卡法筛选和鉴定不规则抗体。结果:在初筛为RhD阴性的633例样本中,检出ccdee 358例(56.56%)、Ccdee 202例(31.91%)、ccdEe 34例(5.37%)、CCdee 20例(3.16%)、CcdEe 13例(2.05%)、ccdEE 3例(0.47%)、CCdEe 2例(0.32%)、CcdEE 1例(0.16%)、CCdEE 0例;在633例初筛RhD阴性样本中,检出抗-D抗体阳性者44例,各表型抗-D抗体例数及占各自总数的比例为ccdee 29例(8.10%),Ccdee 12例(5.94%),ccdEe 2例(5.88%),CedEe 1例(7.69%)。结论:西安地区RhD阴性住院患者Rh血清学表型以ccdee为主,初筛RhD阴性个体抗-D抗体的产生与是否含有C或E抗原无关。

Objective:To statistically analyze the distribution of Rh phenotype and the detection of irregular antibodies in primary screening RhD negative hospitalized patients in Xi’an area,and study the correlation between different phenotypes and anti-D antibody production. Method:Rh serotype phenotypes were identified by Rh blood typing card in 633 primary screening RhD negative hospitalized patients,and irregular antibodies were screened and identified by indirect anti-human globulin microcolumn gel card. Result:Among 633 cases of primary screening RhD negative samples,358 cases(56.56%)were ccdee phenotype,202 cases(31.91%)were Ccdee phenotype,34 cases(5.37%)were ccdEe phenotype,20 cases were CCdee phenotype(3.16%),13 cases were CcdEe phenotype(2.05%),3 cases were ccdEE phenotype(0.47%),2 cases were CCdEe phenotype(0.32%),1 case was CcdEE phenotype(0.16%),and none was CCdEE phenotype.Among 633 cases of primary screening RhD negative samples,44 cases were positive for anti-D antibody.The number of anti-D antibodies in each phenotype and the proportion of the total number were 29 cases(8.10%)of ccdee phenotype,12 cases(5.94 %)of ccdee phenotype,2 cases(5.88 %)of ccdee phenotype,and 1 case(7.69 %)of CedEe phenotype.Conclusion:The Rh serotype phenotype of RhD-negative hospitalized patients in Xi’an is mainly ccdee phenotype,and the production of anti-D antibody in the primary screening RhD-negative individuals is not related to the presence of C or E antigen.