不同方法对夏南牛口蹄疫免疫抗体水平检测的比对试验

A Comparative Test of Different Methods for the Detection of Immune Antibody Levels Against Foot and Mouth Disease in Xianan Cattle

[目的]为探索中小规模养牛场自繁自育条件下,繁殖母牛(指经产1胎以上的母牛,下同)、断奶犊牛(指4月龄以上的犊牛,下同)口蹄疫适宜的免疫时间,检测口蹄疫免疫的效果,为中小规模养牛场制定科学的口蹄疫免疫程序提供参考数据,开展本试验。[方法]采用液相阻断ELISA、固相竞争ELISA两种检测方法进行比对试验。[结果]采用液相阻断ELISA试验结果显示:繁殖母牛抗体效价,在免疫后30 d,60 d,90 d和120 d时免疫抗体合格率分别为65.6%,87.5%,78.1%和46.8%;断奶犊牛在免疫后10 d,40 d,70 d,100 d时免疫抗体合格率分别为75%,100%,83.3%,41.6%。采用固相竞争ELISA试验结果显示:繁殖母牛抗体效价,在免疫后30 d,60 d,90 d,120 d时免疫抗体合格性率分别为78.1%,100%,100%,75%;断奶犊牛在免疫后10 d,40 d,70 d,100 d时免疫抗体合格率分别为83.3%,100%,100%,75%。[结论]从试验结果来看,固相竞争ELISA试验检测只能判定结果是抗体阳性、或抗体阴性,试验操作简单方便,结果判定也容易辨别,但与液相阻断ELISA试验结果相比,抗体水平检测有一定的误差。

[Objective]To explore middle and small scale cattle from numerous bred under the condition of agriculture,breeding cows(refers to the production of more than one child cow,similarly hereinafter),weaning calf(refers to the calf of more than 4 months of age,the same below)of the disease of appropriate immune time,detection of FMD immune,for small and medium-sized cattle science of foot-and-mouth disease immunization programs to provide the reference data,to carry out the test.[Methods]liquid blocking ELISA and solid phase competitive ELISA were used for comparison test.[Results]The results of liquid phase blocking ELISA showed that the qualified rate of antibody was 65.6%,87.5%,78.1%and 46.8%at 30,60,90 and 120 days after immunization.At 10,40,70 and 100 days after immunization,the qualified rate of immune antibody of weaned calves was 75%,100%,83.3%and 41.6%,respectively.The results of solid-phase competitive ELISA showed that the qualified rate of antibody was 78.1%,100%,100%and 75%at 30,60,90 and 120 days after immunization.At 10,40,70 and 100 days after immunization,the qualified rate of immune antibody of weaned calves was 83.3%,100%,100%and 75%,respectively.[Conclusion]From the test results,the solid phase competitive ELISA test can only determine the antibody positive or negative,the test is easy to operate,the result is easy to distinguish,but compared with the liquid phase blocking ELISA test results,the antibody level detection has a certain error.