TNF-α对牙周膜成纤维细胞ephrinB2/EphB4表达量的影响

Effect of TNF-αon Expression of ephrinB2/EphB4 in Periodontal Fibroblasts

目的:检测不同浓度TNF-α对牙周膜成纤维细胞ephrinB2/EphB4表达量的影响。方法:收集因正畸需要拔除的健康牙齿,培养原代牙周膜成纤维细胞;用不同浓度TNF-α分别刺激24 h,48 h后检测牙周膜细胞ephrinB2/EphB4 mRNA及蛋白表达量。结果:24 h组ephrinB2与EphB4 mRNA浓度均降低,差异有统计学意义(P<0.05);48 h低浓度组对ephrinB2 mRNA表达量无影响,0.1μg/L使EphB4 mRNA表达量增加,高浓度组使ephrinB2/EphB4 mRNA表达量下降;24 h组,TNF-α浓度为0.1~1μg/L时,ephrinB2/EphB4蛋白表达量无明显变化,TNF-α浓度为10~1000μg/L时ephrinB2/EphB4蛋白表达量下降;48 h组,0.1μg/L TNF-α作用后使EphB4蛋白表达量上升,ephrinB2蛋白表达量无明显变化,1μg/L作用后,ephrinB2/EphB4蛋白表达量均无明显变化,10~1000μg/L刺激后ephrinB2/EphB4蛋白表达量均下降。结论:24 h时TNF-α使ephrinB2/EphB4 mRNA与蛋白表达量均降低,但在48 h时,低浓度作用下EphB4表达量上升,是否可以为低浓度炎症状态下骨改建的研究提供参考。

Objective:To investigate the effects of TNF-αon the expression of ephrinB2/EphB4 mRNA and protein levels in periodontal fibroblasts.Methods:Healthy teeth which need to be extracted due to orthodontic treatment were collected and the primary periodontal fibroblasts were cultured.The expressions of ephrinB2/EphB4 mRNA and protein levels were detected after the periodontal ligament fibroblasts were stimulated with different concentrations of TNF-αafter 24 h and 48 h.Results:The mRNA concentrations of ephrinB2 and EphB4 were both decreased after 24 h(P<0.05).After 48 h,the low concentration group(0.1μg/L-1μg/L)had no effect on ephrinB2 expression.0.1μg/L could increase the expression of EphB4.However,the high concentration could decrease the expression of ephrinB2/EphB4.Conclusion:ephrinB2/EphB4 was decreased by TNF-αafter 24 h,however,EphB4 expression was increased after 48 h,which was implied to be a reference to study the bone remodeling in low-concentration inflammatory state.