过表达IGFBP3基因抑制肾癌细胞增殖及侵袭促进凋亡

Overexpression of IGFBP3 inhibits the proliferation and invasion of renal cancer cells and promotes apoptosis

目的研究过表达胰岛素样生长因子结合蛋白3(IGFBP3)基因对肾癌细胞增殖、迁移能力及凋亡情况的影响。方法分别将肾癌细胞系ACHN和786-O分为Control组(转染pSectag-A空白质粒)及IGFBP3组(转染pSectag-IGFBP3质粒)。用Western blot法检测上述2种细胞上清液中IGFBP3蛋白的表达情况。转染48h后,采用MTT法、流式细胞术检测细胞的增殖和凋亡情况。采用划痕实验检测细胞的迁移情况。采用Transwell小室检测细胞的侵袭能力。结果转染pSectag-IGFBP3质粒可显著提高ACHN及786-O细胞中IGFBP3的蛋白表达水平。过表达IGFBP3基因后,与Control组相比,IGFBP3组细胞的增殖能力减弱,凋亡率显著增加,差异有统计学意义(均P<0.05);IGFBP3组细胞的迁移和侵袭能力明显减弱,差异有统计学意义(均P<0.05)。结论过表达IGFBP3可以抑制肾癌细胞的增殖和侵袭能力,促进细胞凋亡。

Objective To investigate the effects of overexpression of insulin-like growth factor binding protein 3(IGFBP3)gene on the proliferation,migration and invasion abilities and apoptosis of renal cancer cells.Methods Renal cancer cell lines ACHN and 786-O were divided into a control group(transfected with pSectag-A blank plasmid)and an IGFBP3 group(transfected with pSectag-IGFBP3 plasmid).Western blot was used to detect the expression of IGFBP3 protein in the supernatant of the cells.Then,48 h after transfection,MTT and flow cytometry were adopted to detect cell proliferation and apoptosis.The wound healing assay was used to determine cell migration ability.Transwell assay was used to detect cell invasion ability.Results Transfection with pSectag-IGFBP3 plasmid significantly increased the level of IGFBP3 protein in ACHN and 786-O cells.After overexpression of IGFBP3 gene,compared with the control group,the IGFBP3 group presented weakened proliferation abilities,with an increased apoptotic rate(P<0.05).Furthermore,the IGFBP3 group also showed remarkably reduced proliferation and invasion abilities(P<0.05).Conclusions IGFBP3 overexpression can inhibit the abilities of proliferation and invasion of renal cancer cells and promote apoptosis.