ADCY2基因rs10462841位点多态性与双相障碍治疗效果的关联研究

Association of ADCY2 gene rs10462841 polymorphism with the therapeutic effect of bipolar disorder

目的探讨腺苷酸环化酶2(ADCY2)基因rs10462841多态性与双相障碍治疗效果的关联性。方法以99例双相障碍患者为研究对象,运用大体评定量表(GAS)评估病情严重程度和疗效,采用PCR技术检测ADCY2基因rs10462841位点基因分型。结果ADCY2基因rs10462841位点检测出C、G 2种等位基因,共CC、CG、GG 3种基因型。不同基因型患者的性别、首次发病年龄、首次发病临床相等临床特征分布的差异无统计学意义(P>0.05);不同基因型患者疗效的差异无统计学意义(P>0.05)。从不同时点看,治疗前和治疗第4、8、12周末,不同基因型患者GAS评分的差异无统计学意义(P>0.05);治疗第1周末和第2周末,不同基因型患者GAS评分比较,CC型>CG型>GG型,差异有统计学意义(P<0.05)。结论ADCY2基因rs10462841位点多态性对双相障碍患者的远期疗效无显著影响,与早期疗效存在关联性,了解其基因多态性可指导双相障碍患者的临床个体化用药。

Objective To investigate the association of rs10462841 loci polymorphism of ADCY2 gene with the therapeutic effect for patients with bipolar disorder.Methods A total of 99 patients with bipolar disorder were included in this study.The severity and therapeutic effect of the disease were assessed by the Global Assessment Scale(GAS).Polymerase chain reaction(PCR)and DNA sequencing analysis were used to detect the genotype of rs10462841 loci of ADCY2 gene.Results Two alleles of Cand G,and three genotypes of CC,CG and GG were detected at rs10462841 of ADCY2 gene.There was no significant difference in the distribution of gender,age of first onset and clinical characteristics of first onset among patients with different genotypes(P>0.05).There was no significant difference in the therapeutic effect of patients with different genotypes(P>0.05).There was no significant difference in GAS scores of different genotypes before treatment and at the end of the 4 th,8 th and 12 th weeks(P>0.05).At the end of the first and the second week,the GAS scores of different genotypes were statistically significant(CC>CG>GG,P<0.05).Conclusions The genetic polymorphisms of ADCY2 gene rs10462841 may have no significant effect on the long-term efficacy of patients with bipolar disorder.It is related to the early efficacy of treatment.Understanding its gene polymorphism can guide the clinical individualized medication of bipolar disorder.