摘要
以土茯苓6种黄酮类成分为底物,研究了不同孵育时间、不同CYP3A4酶浓度对6种成分代谢的影响。通过土茯苓6种黄酮类成分的酶促动力学,得到米氏常数(Km)、最大反应速度(Vmax)和代谢清除率(Clint)。结果表明,以表儿茶素、新落新妇苷、落新妇苷、异落新妇苷、黄杞苷、白藜芦醇为底物时,Km分别为1.39,1.38,2.37,1.59,0.79,2.01μmol/L;Vmax分别为0.02,0.01,0.02,0.02,0.01,0.02μmol/(L·min);Clint分别为(1.14,0.96,0.90,0.99,1.42,0.97)×10^-2min^-1。
Six flavonoids of Smilax glabra were used as substrates to study the effects of different incubation time and different concentrations of CYP3 A4 on the metabolism of six components.The Michaelis constant(Km),the maximum reaction rate(Vmax) and the metabolic clearance rate (Clint) were obtained from the enzymatic kinetics of six flavonoids from Smilax glabra.The results showed that Kmwere 1.39,1.38,2.37,1.59,0.79, 2.01μmol/L,Vmax were 0.02,0.01,0.02,0.02,0.01,0.02 μmol/(L·min),and Clintwere(1.14,0.96,0.90, 0.99,1.42,0.97)×10^-2 min^-1 for epicatechin,neoastilbin,astilbin,isoastilbin,engeletin and resveratrol as substrates,respectively.
作者
陈荣达
谢静
史玉蕊
刘桂明
郑梅竹
李丽
CHENRongda;XIE Jing;SHI Yurui;LIU Guiming;ZHENG Meizhu;LI Li(College of Chemistry,Changchun Normal University,Changchun 130032)
出处
《分析试验室》
CAS
CSCD
北大核心
2020年第7期760-765,共6页
Chinese Journal of Analysis Laboratory
基金
国家自然科学基金(81373899)
吉林省科技厅项目(20180101153JC,JJKH20181168KJ)
长春师范大学研究生创新项目(Cscxy2018001,Cscxy2017025)资助。