PCL/ZrO2骨组织工程支架3D打印制备方法及其性能研究

Preparation and properties of PCL/ZrO2 bone tissue engineering scaffold based on 3D printing

目的为制备出满足骨缺损修复需要的具有一定力学强度和生物活性的骨组织工程支架,本文选取聚己内酯(polycaprolactone,PCL)和纳米氧化锆(ZrO2)粉末制备出三维多孔复合材料支架。方法采用高温熔融挤出3D打印方式制备PCL/ZrO2复合材料支架,为获取支架的几何形态、力学性能和生物学性能,利用扫描电子显微镜(scanning electron microscope,SEM)和万能试验机(material test system,MTS)分别分析了支架的形貌和压缩性能,并通过体外细胞培养的方式测试复合材料支架的生物相容性。结果制备完成的复合材料支架具有良好的三维孔隙结构,孔径≥400μm,孔隙率≥40%。对比纯PCL支架,PCL/ZrO2复合材料支架的力学性能显著提高,杨氏模量提高0.4倍左右,抗压强度提高0.5倍左右。在体外实验中,细胞培养7 d后PCL/ZrO2复合材料支架上的细胞增殖对比纯PCL支架有显著提高。结论基于该结果,本文制备出的PCL/ZrO2生物活性骨组织支架在骨组织工程方面有一定的应用前景。

Objective In order to prepare bone tissue engineering scaffolds with certain mechanical strength and biological activity to meet the needs of bone defect repair,three-dimensional porous composite scaffolds were prepared by using polycaprolactone(PCL)and nano-zirconia(ZrO2)powder.Methods The PCL/ZrO2 composite material support was prepared by high temperature melt extrusion 3D printing.The geometry,mechanical properties and biological properties of the scaffolds were obtained.The morphology and compression properties of the scaffolds were analyzed by scanning electron microscope(SEM)and material test system(MTS).The biocompatibility of the composite scaffold was tested by in vitro cell culture.Results The prepared composite scaffold had a good 3D pore structure,a pore diameter of≥400μm,and a porosity of≥40%.Compared with the pure PCL stent,the mechanical properties of the PCL/ZrO2 composite stent were significantly improved,the Young’s modulus was increased by about 0.4 times,and the compressive strength was increased by about 0.5 times.In vitro,cell proliferation on the PCL/ZrO2 composite scaffold was significantly improved after 7 days of cell culture compared to the pure PCL scaffold.Conclusions Based on the results,the PCL/ZrO2 bioactive bone tissue scaffold prepared in this paper has certain application prospects in bone tissue engineering.