摘要
Cytosine methylation is an epigenetic modification of DNA that can affect gene expression and silence transposable element(TE)activities.In plants,cytosine methylation occurs in three distinct sequence contexts:CG,CHG,and CHH(where H=C,A,or T)(Law and Jacobsen,2010).The RNA-directed DNA methylation(RdDM)pathway catalyzes all types of de novo cytosine methylation,especially within small TEs or at the edges of large TEs.In brief,two plant-specific RNA polymerases,Pol IV and Pol V,independently synthesize relatively short single-stranded RNAs at RdDM target regions(Law and Jacobsen,2010).Pol IV transcripts are converted to double-stranded RNA by RNA-DEPENDENT RNA POLYMERASE 2.These double-stranded RNAs are processed into 24-nt small interfering RNAs(siRNAs)by DICER-LIKE 3.ARGONAUTE 4 binds these siRNAs and is targeted to RdDM target loci,with Pol V transcripts as scaffolds.Finally,DOMAINS REARRANGED METHYLTRANSFERASE 2 is recruited to RdDM target regions through an indirect interaction with ARGONAUTE 4 and catalyzes cytosine methylation.
基金
This work was supported by JSPS KAKENHI(19H04873).