通辽市家畜感染无形体属病原体的调查

Investigation of Anaplasma species infected by livestock in Tongliao

目的调查内蒙古通辽地区绵羊和牛的无形体感染情况。方法采用PCR方法检测内蒙古通辽市扎鲁特旗及科左中旗散放76只绵羊和20头牛的血样本,扩增嗜吞噬细胞无形体(Anaplasma phagocytophilum)、中央无形体(A.centrale)和牛无形体(A.bovis)的16 S rRNA基因和羊无形体(A.capra)的gltA基因。结果76只绵羊血样本中37份扩增到嗜吞噬细胞无形体目的基因,阳性率为48.7%;76只绵羊血样本中16份扩增到中央无形体目的基因,阳性率为21.1%。所有绵羊的血样本均未扩增到羊无形体和牛无形体的目的基因,20头牛的血样本均未扩增到4种无形体的目的基因。结论通辽地区羊存在嗜吞噬细胞无形体和中央无形体感染,提示该地区存在无形体病的自然疫源地和可能存在人无形体病。

To investigate Anaplasma spp. infection in the sheep and cow from Jarud Banner and Horqin Left Middle Banner of Tongliao city, Inner Mongolia. Blood samples of 76 sheep and 20 cows were tested by PCR to amplify the 16S rRNA genes of Anaplasma phagocytophilum, A. centrale, A. bovis and gltA genes of A. capra. The test result shows that 37 out of 76 sheep blood samples have been amplified with the A. phagocytophilum target gene, with a positive rate of 48.7%;16 of 76 sheep blood samples were amplified with the A. centrale target gene, with a positive rate of 21.1%. None of the sheep’s blood samples have been amplified with the A.bovis and A.capra target genes, and none of blood samples of 20 cows have been amplified with all four Anaplasma spp. In conclusion, there is A. phagocytophilum and A. centrale infection existing in sheep from Tongliao region, furthermore, there is potential existence of a natural epidemic source of anaplasmosis and the risk of Human granulocytic anaplasmosis in the region.