响应面优化水酶法提取奇亚籽油工艺

Optimization of aqueous enzymatic extraction of chia seed oil by response surface methodology

以奇亚籽为原料,采用水酶法提取奇亚籽油。在单因素实验的基础上,采用响应面法对水酶法提取奇亚籽油的工艺条件进行优化。结果表明,水酶法提取奇亚籽油的最佳工艺条件为:碱性蛋白酶作为酶解用酶,酶解温度45℃,液料比8.47∶1,pH 10,酶添加量5.17%,酶解时间2.16 h。在最佳条件下,奇亚籽油提取率为89.53%。

Chia seed was used as raw material and chia seed oil was extracted by aqueous enzymatic method.On the basis of single factor experiment,response surface methodology was used to optimize the extraction process of chia seed oil.The results showed that the optimal conditions for the extraction of chia seed oil were obtained as follows:alkaline protease as hydrolysis enzyme,enzymolysis temperature 45℃,ratio of liquid to material 8.47∶1,pH 10,enzyme dosage 5.17%and enzymolysis time 2.16 h.Under the optimal conditions,the extraction rate of chia seed oil was 89.53%.