MMTV-PyMT乳腺癌小鼠模型肿瘤发生的特点

Characteristics of Tumorigenesis in MMTV-PyMT Breast Cancer Mouse Model

以MMTV-PyMT乳腺癌小鼠模型为对象,研究其肿瘤发生特点。观察MMTV-PyMT转基因小鼠,记录乳腺癌小鼠肿瘤的发病时间、发生位置以及肿瘤小鼠的生存时间,并且通过病理学切片观察不同时间段乳腺癌的病理变化。大多数MMTV-PyMT雌性小鼠在出生后的第8周开始发生肿瘤,而雄性小鼠在第19周左右产生肿瘤;雌鼠自发产生肿瘤后最长生存时间为12周,而雄鼠则为28周。不同时间段的MMTV-PyMT雌性小鼠乳腺肿瘤染伊红染色结果有着明显的区别,早期肿瘤均为浸润性导管癌,随着癌症的发展,癌细胞开始突破基底膜,癌细胞扩散至整个乳腺组织中,整体呈现出弥漫性分布、癌巢着色较深,细胞间推挤或重叠。MMTV-PyMT自发性乳腺癌小鼠雌性小鼠和雄性小鼠均可发生乳腺癌,雌性小鼠具有发病时间早,生存时间短,肿瘤时期变化明显等特点。

In MMTV-PyMT mouse model of breast cancer as the object to study the tumorigenesis characteristics.Observe the MMTV-PyMT transgenic mice,record the onset time,location of tumors and survival time of tumor mice in breast cancer mice,and observe the pathological changes of breast cancer in different periods by pathological sectioning.Most MMTV-PyMT female mice begin to develop tumors in the 8th week after birth,while male mice develop tumors around the 19th week;the longest survival time for female mice after spontaneous tumor development is 12 weeks,while for male mice it is 28 weeks.The results of HE staining of breast tumors in MMTV-PyMT female mice at different periods are significantly different.The early tumors were invasive ductal carcinomas.As cancer progressed,the cancer cells began to break through the basement membrane,and the cancer cells spread to the entire breast tissue.Medium,the whole showed a diffuse distribution,the color of the cancer nest was dark,and the cells pushed or overlapped.MMTV-PyMT spontaneous breast cancer mice can occur in both female and male mice.Female mice have the characteristics of early-onset time,short survival time,and obvious changes in tumor stage.