不同体色草金鱼鳞片墨蝶呤还原酶mRNA表达水平研究

The expression levels of sepiapterin reductase mRNA in different color scales of Carassius auratus

为探究墨蝶呤还原酶(sepiapterin reductase,SPR)基因在草金鱼(Carassius auratus)体色形成中的作用,本研究利用半定量反转录聚合酶链式反应(Semi-QRT-PCR)技术,对spr基因在不同体色草金鱼鳞片的mRNA表达水平进行了研究。结果显示,spr在草金鱼不同颜色鳞片中表达程度不同,且在黄色、黑色鳞片中表达量较高。本实验初步探究了spr与草金鱼体色形成的关系,旨在为进一步探究spr在体色形成中的作用提供参考资料。

In order to investigate the role of spr gene in body color formation of Carassius auratus,semi-QRT-PCR was used to study the mRNA expression level of spr gene in scales of goldfish with different body colors.The results showed that the expression level of spr was different in different color scales of grass goldfish,and the expression level was higher in yellow scales and black scales.This experiment preliminarily explored the relationship between spr and body color formation of grass goldfish,and aimed to provide reference for further exploring the role of spr in body color formation.