新型大孔径介孔二氧化硅吸附牛血清蛋白的性能研究

Study on the Adsorption Properties of Large-pore Mesoporous Silica for Bovine Serum Albumin

创新地利用一步法成功合成了大孔径介孔二氧化硅(large-pore mesoprous silica,LPMS)。采用多种测试手段对LPMS的形貌、结构及热稳定性进行了表征。结果表明,LPMS具有较大的孔径(22.8 nm)和较高的孔容(2.35 cm^3/g),同时有机模板在合成过程中已被完全去除,简化了制备工艺,保留了大量的硅羟基。选择牛血清蛋白作为对象对LPMS的蛋白吸附能力进行研究,结果表明LPMS对蛋白的吸附容量为193.2 mg/g,并且符合二级动力学吸附模型和Freundlich等温吸附模型,属于多分子层化学吸附。

A large-pore mesoprous silica(LPMS)was firstly synthesized by one-step method.The morphology,structure and thermal stability of LPMS were characterized by using various test methods.Results showed that LPMS had a large mesopore size(22.8 nm)and a high pore volume(2.35 cm^3/g),more importantly,the organic template was completely removed during the synthesis process,the synthesis process was simplified,and a large amount of silicon hydroxyl groups was retained.Bovine serum albumin(BSA)was selected as the target to study the protein adsorption capacity of LPMS.The results showed that the protein adsorption capacity of LPMS was 193.2 mg/g,and it was fit with the second-order kinetic adsorption model and Freundlich isotherm adsorption model,which belonged to multi-molecular layer chemical adsorption.