2种奶牛隐性乳房炎检测方法比较

Comparison of Two Methods for Detecting Recessive Mastitis in Cows

文章旨在更准确地检测隐性乳房炎,为评价隐性乳房炎乳汁的变化提供参考。试验分别采用加州乳房炎检测(CMT)法与溴麝香草酚蓝检测(BTB)法对35头奶牛进行隐性乳房炎的检测,同时比较了2种测定方法下乳pH、乳血清淀粉样蛋白A(SAA)、乳成分及血液常规成分的差异。结果表明,2种检测方法中,CMT法检出阳性率(40%)高于BTB法(34.3%),但差异不显著(P>0.05);而CMT法的吻合率(35.71%)低于BTB法(41.67%)。2种检测方法呈阳性的乳pH显著高于阴性组(P<0.05),BTB检测法呈阳性乳的体细胞数显著高于(P<0.05)阴性乳,而CMT法检测呈阳性的乳中体细胞数(SCC)的对数值比阴性乳高13.6%;BTB法检测呈阳性的SAA显著低于阴性组(P<0.05)。2组阳性与阴性组间奶牛血细胞成分无显著差异(P>0.05)。结论表明,2种检测方法中,CMT法比BTB法灵敏,但BTB法更准确。

The aim of this paper was to detect recessive mastitis more accurately and to provide a reference for evaluating the change of recessive mastitis milk.The methods of California mastitis test(CMT)and bromine vanilla phenol blue test(BTB)were used in the present experiment to detect the recessive mastitis of 35 cows,and the pH,milk serum amyloid A(SAA),milk composition and blood cells composition were also detected and compared between two methods.The results showed that the positive rate detected by CMT method(40%)was higher than that of BTB method(34.3%),while the difference was not significant(P>0.05),and the match rate of CMT method(35.71%)was lower than that of BTB method(41.67%).The pH of milk in two positive groups were significantly higher(P<0.05)than that of negative group.The logarithms of somatic cell count of positive group detected by BTB test was significantly higher than that of negative group(P<0.05),which was numerically lower than that positive group detected by CMT test.The SAA content in the milk of positive group detected by BTB test was significantly lower than that of the negative group(P<0.05).There was no significant difference in the blood cell components among the two testing positive and negative groups(P>0.05).It was concluded that the CMT test was more sensitive than that of BTB test,while the BTB test was more accurate.