PD-1/PD-L1抑制剂活性筛选模型的建立与研究

PD-1/PD-L1 assay and screening for its inhibitor from plants

肿瘤免疫治疗是继手术、放疗和化疗之后第4种有效的癌症治疗方法,免疫检查点阻断疗法是其中最有效的策略之一.PD-1/PD-L1是目前最受关注的免疫检测点(immune checkpoint)蛋白,以其为代表的免疫检测点抗体抑制剂已经应用到临床并取得良好的治疗效果.本研究针对PD-1/PD-L1相互作用,用ELISA方法建立了PD-1/PD-L1抑制剂活性筛选模型,并用已知抑制剂对实验模型的有效性进行了验证.最后利用该模型对32个植物来源的天然产物样本进行了活性筛选,发现其中2个来自于苍耳和落葵薯的提取物样品对PD-1/PD-L1有一定抑制作用.

Following surgery, radiotherapy and chemotherapy, tumor immunotherapy is regarded as the fourth effective therapy for cancers, and the treatment for blocking immune checkpoint is one of the most effective strategies in tumor immunotherapy. PD-1/PD-L1 is the most concerned immunocheckpoint protein at present, and its representative immunocheckpoint antibody inhibitors have been applied in clinical practice and achieved good therapeutic effects. In this study, the screening assay for PD-1/PD-L1 inhibitors was established by ELISA method, and the validity of the assay was verified by the known inhibitor. Finally, the inhibitory activity of 32 natural products from plants was screened by using this assay, and two extract samples from Xanthium sibiricum Patr. and Anredera cordifolia were found to exhibit certain inhibition on PD-1/PD-L1.