正交法优化黄精地上茎叶中有效成分的提取工艺

Optimization of extraction technology of effective components from upper stem and leaf of Polygonatum sibiricum by orthogonal method

以黄精(Polygonatum sibiricum)地上部茎叶为试材,采用单因素试验研究了料液比、提取温度、提取时间和乙醇体积分数对提取黄酮、多酚和多糖得率的影响。通过正交试验优化黄酮、多酚和多糖的提取工艺。结果表明,提取黄酮最佳条件为提取温度70℃,料液比1∶80,提取时间2.5 h,乙醇体积分数为90%,黄酮平均得率为4.01%;提取多酚最佳条件为料液比1∶40,提取温度80℃,提取时间1.0 h,乙醇体积分数为60%,多酚平均得率为0.62%;提取多糖的最佳条件为提取温度80℃,料液比1∶20,提取时间2.0 h,多糖平均得率为18.61%。验证试验表明,这3种优化工艺稳定可行。

Using the aboveground stems and leaves of Polygonatum sibiricum as raw materials,the effects of solid-liquid ratio,extraction temperature,extraction time and ethanol volume fraction on the yield of flavonoids,polyphenols and polysaccharides were studied by single factor experiment.The extraction process of flavonoids,polyphenols and polysaccharides was optimized by orthogonal test.The results showed that the optimum conditions for extracting flavonoids were the extraction temperature 70℃,solid-liquid ratio 1∶80(m∶m),extraction time 2.5 h,ethanol volume fraction 90%,average yield of flavonoids was 4.01%.The optimum conditions for extracting polyphenols were the extraction temperature 80℃,material-liquid ratio 1∶40,extraction time 1.0 h,ethanol volume fraction 60%,average yield of polyphenols was 0.62%.The optimum conditions for polysaccharide extraction were the extraction temperature 80℃,solid-liquid ratio 1∶20,extraction time 2.0 h,and the average yield of polysaccharide was 18.61%.The test results showed that the three optimized processes were stable and feasible.