摘要
节律钟输出基因GIGANTEA通过光周期途径促进植物开花,为研究火龙果GIGANTEA同源基因功能,应用RT-PCR克隆获得该基因的开放阅读框序列,命名为Hylocereus polyrhizus GIGANTEA(HpGI),GenBank登录号为MK609546。序列分析结果表明,HpGI开放阅读框长度为3537 bp,编码1178个氨基酸。系统进化分析显示,HpGI与甜菜BvGI、菠菜SoGI、丝石竹GpGI的分子进化距离较近。预测HpGI定位于细胞核,无信号肽和跨膜螺旋结构域,属于非分泌蛋白。基因表达分析结果表明,HpGI基因在茎和花芽中的表达量显著高于茎芽、果皮和根;相比对照,延长光周期处理8 d时,其表达量显著上调。推测HpGI基因可能通过光周期途径促进火龙果开花。
Rhythms clock output gene GIGANTEA promotes flowering via the photoperiod pathway.To analysis the function of GIGANTEA homologous gene in pitaya[Hylocereus polyrhizus(Weber)Britton&Rose],the open reading frame(ORF)sequence was cloned through RT-PCR.The ORF of Hylocereus polyrhizus GIGANTEA(HpGI)is 3537 bp,encoding 1178 amino acids,and the GenBank accession number is MK609546.The phylogenetic analysis showed that HpGI and Beta vulgaris GIGANTEA are the closest in molecular evolution distance,followed by Spinacia oleracea GIGANTEA and Gypsophila paniculate GIGANTEA.It was speculated that HpGI was located in the nucleus,and it was a non-secretory protein.No signal peptide and transmembrane helix domain were found.The results of quantitative RT-PCR revealed that the expression level of HpGI gene in the stem and flower bud was significantly higher than that in the stem bud,peel and root.Moreover,the transcription of the HpGI gene was significantly induced by the 8 d night-breaking treatment,compared to the untreated samples.It was deduced that HpGI may promote flowering via the photoperiod pathway in pitaya.
作者
肖图舰
马玉华
袁启凤
解璞
毛永亚
严佳文
廖仕琴
XIAO Tujian;MA Yuhua;YUAN Qifeng;XIE Pu;MAO Yongya;YAN Jiawen;LIAO Shiqin(Institute of Pomology,Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550006,China;Fruit Industry Development Office of Luodian County,Luodian,Guizhou 550100,China)
出处
《热带作物学报》
CSCD
北大核心
2020年第7期1298-1304,共7页
Chinese Journal of Tropical Crops
基金
贵州省重点科技攻关项目(黔科合NZ[2015]3001号)
贵州省科技计划项目(黔科合平台人才[2017]5603)
贵州省农业动植物育种项目(黔农育专字[2017]010号)。