水稻黄叶不育系突变体H08S的表型特征与遗传分析

Phenotypic Characteristics and Genetic Analysis of a Rice PTGMS Line Mutant H08S with Yellow Leaf

光温敏不育系的育性因易受光温条件影响而出现波动,导致种子纯度不够,是水稻生产上常见的问题。本研究对1个水稻黄叶不育系突变体H08S进行温度敏感性分析和叶绿体超微结构观察,同时通过构建分离群体进行突变基因的遗传模式分析。结果表明,突变体H08S的表型受温度影响,为低温表达型叶色突变体,且其叶绿体结构出现异常,表现类囊体的片层结构减少,说明H08S基因的突变影响叶绿体的正常发育。突变体H08S分别与‘日本晴’、‘02428’构建F2群体和BC1F1群体,观察并统计群体植株叶色表型的分离情况,并进行卡方检验,结果表明该突变性状受1对隐性单基因控制。

PTGMS lines are susceptible to light and temperature conditions during their fertility sensitive period,which results in fertility fluctuation and seriously affects the purity of the original or hybrid seeds of the male sterile lines.In this paper,the temperature sensitivity and chloroplast ultrastructure of a rice PTGMS mutant H08S with yellow leaf were analyzed,and the genetic model of the mutant gene was analyzed by constructing two segregated populations.The results showed that the phenotype of mutant H08S was affected by temperature,and it was a low temperature expression type of leaf color mutant.The chloroplast structure of mutant H08S was abnormal,and the lamellar structure of thylakoid was reduced.This indicated that the mutation of H08S gene affected the normal development of chloroplast.F2 population and BC1F1 population were constructed with mutant H08S and‘Nipponbare’and‘02428’,respectively.The isolation of leaf color phenotypes was observed and counted,and chi-square test was carried out.The results showed that the mutant trait was controlled by a pair of recessive single genes.