澳系睡莲花粉离体萌发及低温保存研究

Pollen Germination in vitro and Cryopreservation Reserch of Anecphya Waterlily

为研究适宜睡莲花粉离体萌发的培养基和低温保存的方法,以睡莲品种‘默笙’和3份原种睡莲的花粉为试验材料,采用单因素、正交试验设计,筛选出最适宜的培养基,观测睡莲花粉在不同温度下保存的萌发率。结果表明,4种澳系睡莲花粉的最适培养基的各组分浓度不同,花粉的生活力存在差异,澳洲原生浅色睡莲与澳洲原生深色睡莲的花粉生活力较高,分别为43.90%和45.63%;‘默笙’睡莲的花粉生活力次之,为31.84%;‘白巨睡莲’的花粉生活力较低,为9.73%;4种澳系睡莲花粉均不耐贮藏,不同温度下保存72 h后萌发率均为0。本研究结果可为睡莲杂交育种的亲本选择、花粉的低温保存研究提供参考。

The pollens of Nymphaea‘Moshen’and three orginal Nymphaea accessions were used as the test material to study the optimal medium components for the pollen germination in vitro of Nymphaea and the method for prolonging pollen viability at low temperature.The single factor and orthogonal design were used to select the most suitable medium,and observe the germination rate of the pollens preserved at different temperatures.The optimum medium concentration for the pollens of the four accessions was different.The pollen viability varied among accessions.The pollen viability of N.immutabilis subsp.Immutabilis blue form and N.immutabilis subsp.immutabilis purple form was 43.90%and 45.63%respectively,and that of N.‘Moshen’and N.‘Gigantea Albertde Lestang’was 31.84%and 9.73%,relatively.The pollen of Nymphaea was not tolerant to storage,and lost the viability after 72 hours of storage at different temperatures.The results of the study could provide references for parents selection and pollen cryopreservation in hybrid breeding of Nymphaea.