碳离子束辐照对火龙果种子萌发、存活率及幼苗生长的影响

Effect of Carbon Ion Beams Irradiation on Seed Germination,Survival and Seedling Growth of Hylocereus polyrhizus

为探究碳离子束辐照对火龙果种子的损伤效应并且确定适宜的诱变辐照剂量,以红肉火龙果种子为试材,采用5个不同剂量(0、15、30、45、60 Gy)的碳离子束12C6+进行辐照处理,观察其对火龙果种子萌发、存活率及幼苗生长的影响。结果表明,12C6+离子束在辐照剂量0~60 Gy内大多数种子均能正常萌发,对发芽率的影响不大,而对发芽势和发芽指数存在一定的抑制作用;对幼苗苗高具有显著的促进作用,而对根长、子叶长和宽均呈现不同程度的抑制作用。碳离子束辐照对火龙果幼苗存活率的影响具有极显著的剂量效应,随剂量升高而极显著下降。火龙果种子碳离子束辐照的半致死剂量(LD50)为24 Gy,建议适宜辐照剂量范围为15~30 Gy。

In order to explore the damage effect of carbon ion beams irradiation on seeds and determine the suitable mutagenic irradiation doses,the effects of carbon ion beams on seed germination,survival and seedling growth were investigated by irradiating the dry seeds of red pitaya(Hylocereus polyrhizus)at various doses of 12C6+ion beams(0,15,30,45 and 60 Gy).The results showed that most of the seeds could germinate normally at the irradiation doses ranging from 0 to 60 Gy,and the effects of carbon ion beams on the germination rate were not significant,but some certain inhibitory effects on germination potential and germination index were observed.It could significantly promote the shoot height,but inhibit the radicle length,cotyledon length and width to varying degrees.Carbon ion beams irradiation had a very significant dose effect on the survival rate of the seedlings which significantly decreased with the irradiation dose.The semi-lethal dose(LD50)of the seeds of red pitaya irradiated by carbon ion beams was 24 Gy.The recommended irradiation doses is ranged from 15 to 30 Gy.Those results would provide dose references and guidance for the subsequent study of carbon ion beams mutation breeding of pitaya.