血清淀粉样蛋白A1对头颈部鳞状癌细胞HN4增殖迁移能力的影响及其机制研究

Serum amyloid A1 enhances the migration of head and neck squamous cell carcinoma HN4 cells through inducing the expression of CXCR3A

目的探究人血清淀粉样蛋白A1(SAA1)通过调控趋化因子受体3A(CXCR3A)增强人头颈部鳞状癌细胞HN4增殖迁移能力的作用机制。方法本研究通过MTT法以及划痕实验验证SAA1对头颈部鳞状癌细胞HN4的增殖和迁移能力的影响;用实时荧光定量PCR法检测SAA1对CXCR3A表达量的影响;用免疫蛋白印迹法检测Erk1/2,p38 MAPK的磷酸化水平。结果SAA1组(5μmol·L-1和10μmol·L-1)能够明显促进HN4细胞的增殖,且明显提高其迁移能力;SAA1能够诱导HN4细胞表达CXCR3A,且能够诱导丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)信号通路中Erk1/2和p38 MAPK的磷酸化。结论SAA1通过诱导CXCR3A的表达促进头颈部鳞状癌细胞HN4的增殖和迁移,其作用机制可能与激活Erk1/2和p38 MAPK信号通路有关。

Objective To investigate that human serum amyloid A1(SAA1)enhances the migration ability of human head and neck squamous carcinoma(HNSCC)HN4 cells by regulating CXCR3A.Methods The effects of SAA1 on proliferation and migration of HN4 cells were measured by MTT and wound healing experiments.Real-time quantitative PCR was used to detect the effect of SAA1 on CXCR3A expression.The phosphorylation of Erk1/2 and p38 MAPK was detected by Western Blot.Results SAA1 group(5μmol·L-1 and 10μmol·L-1)significantly enhanced the proliferation and cell migration of HN4 cells.SAA1 induced the expression of CXCR3A in HN4 cells and the phosphorylation of Erk1/2 and p38 MAPK.Conclusion Our findings suggested that SAA1 promoted the proliferation and migration of HN4 cells by inducing the expression of CXCR3A,which might be related to the activation of Erk1/2 and p38 MAPK.