木薯种茎劣变的蛋白质组学分析

Proteomics profiling of the deteriorated cassava(Manihot esculenta Crantz) stem

以华南8号(SC8)和南植199(NZ199)种茎为试验材料,分析其在老化0、2、4、6 d的蛋白质表达差异。与老化0 d的种茎相比,老化2、4、6 d的种茎,SC8的差异表达蛋白质总数分别为38、64和68个,上调表达的差异蛋白质数分别为11、18和25个,下调表达的差异蛋白质数分别为27、46和43个;NZ199的差异表达蛋白质总数分别为55、63和84个,上调表达的差异蛋白质数分别为17、23和43个,下调表达的差异蛋白质数分别为38、40和41个。与老化0、2、4 d凝胶相比,老化6 d种茎的凝胶中,SC8、NZ199分别检测到19、36个共有差异蛋白质点,且分别有17、30个被成功匹配;2个品种种茎差异蛋白点功能基本相似,主要涉及分子伴侣、碳水化合物和能量代谢、转移、防御、解毒和抗氧化等相关蛋白质。在成功匹配的差异蛋白质中,D–3–磷酸甘油脱氢酶、磷酸甘油酸激酶、类壳三糖苷酶–1蛋白、苹果酸酶、拟定肉桂醇脱氢酸、1–脱氧–D–木酮糖–5–磷酸还原异构酶等差异蛋白质下调表达;拟定谷胱甘肽硫–转移酶parC、类异黄酮还原酶、病程相关蛋白Bet v I家族、热激蛋白、GPN60蛋白、α–淀粉酶、ATP合成酶β亚基和烯醇化酶等差异蛋白质上调表达。差异表达蛋白质主要参与对氧化应激反应、毒素分解、谷胱甘肽代谢、蛋白质折叠和糖酵解等生物过程,分布于线粒体、叶绿体、溶质、细胞质、细胞壁等位置,主要发挥结合功能、催化活性和氧化还原功能。

To look into theoretical basis of the molecular mechanism of deterioration, we took cassava(Manihot esculenta) stem, cassava stems of South China 8(SC8) and Nanzhi 199(NZ199) varieties at four aging degrees as materials and characterized the differentially expressed proteins(DEPs) in the stems responding to four aging degrees. The results showed that: 1) Compared to the non-aged stems, 38 DEPs of the stems of SC8 with two-day age were up-regulated by 11 and down-regulated by 27, 64 DEPs from the stems of SC8 with four-day age were up-regulated by 18 and down-regulated by 46, and 68 DEPs from the stems of SC8 at six-day age were up-regulated by 25 and down-regulated by 43. 2) Compared to the non-aged stems, 55 DEPs of the NZ199 at two-day age were up-regulated by 17 and down-regulated by 38, 63 DEPs from of NZ199 at four-day age were up-regulated by 23 and down-regulated by 40, and 84 DEPs of NZ199 at six-day age were up-regulated by 43 and down-regulated by 41. 3) Compared to the non-aged stems, two days aged and four days aged treatment, 17 of 19 common DEPs were identified from the age of six days for SC8 stems, while 30 of 36 common DEPs were identified from the age of six days for NZ199 stems. These 30 proteins were grouped into five functional categories, chaperone proteins, carbon metabolism and energy metabolism related proteins, transfer proteins, defense proteins, detoxification and antioxidant related proteins. 4) D-3-phosphoglycerate dehydrogenase, phosphoglycerate kinase, chitotriosidase-1-like, malic enzyme, probable cinnamyl alcohol dehydrogenase, 1-deoxy-D-xylulose-5-phosphate reductoisomerase were down-regulated, while probable glutathione S-transferase parC, isoflavone reductase-like, pathogenesis-related protein Bet v I family, heat shock protein, chaperonin CPN60, alpha amylase, ATP synthase subunit beta and enolase were up-regulated. 5) DEPs mainly participated in biological process, including response to oxidative stress, toxin catabolic process, glutathione metabolic process, protein folding and glycolytic process. The proteins were mainly located in mitochondria, chloroplasts, cytoplasm, cell wall, and etc, and played roles of binding, catalytic activity and oxidoreductase activity.