S100A4和环氧化酶-2的共表达与胰腺癌临床预后的相关性研究

Co-expressions of S100A4 and COX-2 in pancreatic carcinoma and their correlation with prognosis

目的:探讨离子结合蛋白(S100A4)和环氧化酶-2(COX-2)在胰腺癌组织中的共表达及相关性,并分析其与临床病理特征及预后的关系。方法:免疫组织化学染色法检测S100A4和COX-2蛋白在128例胰腺癌组织、11例癌旁正常胰腺组织中的表达,分析两者表达相关性及与临床病理特征和预后的关系。结果:S100A4(+)/COX-2(+)、S100A4(+)/COX-2(-)、S100A4(-)/COX-2(+)及S100A4(-)/COX-2(-)在胰腺癌组织中的高表达率分别为66.4%(85/128)、8.6%(11/128)、5.5%(7/128)和19.5%(25/128),且S100A4和COX-2蛋白的表达及共表达均与胰腺癌肿瘤直径、分化程度、TNM分期、淋巴结是否转移及CA19-9水平相关(P<0.05)。S100A4与COX-2的表达呈正相关性(P<0.05)。Kaplan-Meier生存分析发现,高表达S100A4、COX-2或共表达S100A4/COX-2提示患者术后生存期缩短。Cox模型多因素分析显示,分化程度、临床分期、CA19-9及S100A4/COX-2过表达是影响胰腺癌预后的独立危险因素(P=0.000)。结论:S100A4和COX-2可能协同表达上调并参与胰腺癌的发生、发展。联合检测S100A4和COX-2的表达可以为评估胰腺癌预后提供更准确的参考。

Objective: To detect the co-expressions of S100 A4 and cyclooxygenase-2(COX-2)in pancreatic carcinoma tissues and explore their correlations with the clinicopathological features and prognosis of the patients. Methods: The expressions of S100 A4 and COX-2 in 128 cases of pancreatic carcinoma tissues and 11 cases of non-neoplastic adjacent pancreatic tissues were detected by immunohistochemical method,their correlation with clinicopathological features and and prognosis was analyzed. Results: S100 A4(+)/COX-2(+)、S100 A4(+)/COX-2(-)、S100 A4(-)/COX-2(+) and S100 A4(-)/COX-2(-) were highly expressed in66.4%(85/128)、8.6%(11/128)、5.5%(7/128)and 19.5%(25/128)of the pancreatic carcinoma tissues, respectively. The expression of S100 A4, COX-2 and their co-expression were all correlated with tumer size, differentiation,TNM stages, lymph node metastasis and CA19-9(P<0.05). A strong correlation was found between S100 A4 and COX-2 expressions(P<0.05). Kaplan-Meier survival analysis revealed that high expression of S100 A4, COX-2, or co-expression of S100 A4/COX-2 suggested a shortened postoperative survival. Cox model multivariate analysis showed that the differentiation, TNM stages, CA19-9 and co-expression of S100 A4/COX-2 were independent risk factors for the prognosis of pancreatic carcinoma(P=0.000). Conclusion: The high expression of S100 A4 and COX-2 in pancreatic carcinoma is positively correlated, and the up-regulation of their synergistic expression plays an important role in the occurrence and development of pancreatic carcinoma.Combined detection of the expression of S100 A4 and COX-2 can provide a more accurate reference value for evaluating the prognosis of pancreatic carcinoma.