摘要
[Objectives] Uncertainty in liquid chromatography-mass spectrometry detection of nitrofuran metabolite residues in chicken bone was analyzed to find out the influencing factors. [Methods] According to relevant theories such as Evaluation and expression of uncertainty in measurement,the uncertainty in the results of nitrofuran metabolites in chicken bone was analyzed and calculated combining with mathematical models. [Results] Under the addition amount of 1. 0 μg/kg,the uncertainty of the four nitrofuran metabolites was as follows: nitrofurazone metabolites C(SEM)= 0. 981 μg/kg,U = 0. 070 μg/kg,k = 2;furazolidone metabolites C(AOZ)= 1. 032 μg/kg,U = 0. 061 μg/kg,k = 2;furaltadone metabolites C(AMOZ)= 1. 068 μg/kg,U = 0. 076 μg/kg,k = 2;furadantin metabolites C(AHD)=1. 007 μg/kg,U = 0. 046 μg/kg,k = 2. [Conclusions]The uncertainty in the measurement process mainly comes from the purity of the standards,the preparation process,the sample weight,the number of repeated measurement and the recovery of spiked sample. The research results are of great significance for reducing uncertainty and improving data accuracy and reliability during actual operation.
