28份余甘子品种遗传多样性的ISSR分析及指纹图谱构建

Genetic diversity analysis and DNA fingerprint mapping of 28 varieties of Phyllanthus emblica L.based on ISSR molecular marker

【目的】利用ISSR分子标记技术对28份余甘子种质资源进行遗传多样性分析,并构建其指纹图谱。【方法】从96条ISSR引物中筛选得到9条核心引物,用作广东省农业科学院收集的华南地区28份余甘子品种分子标记,利用UPGMA聚类分析余甘子种质资源遗传多样性,以核心引物UBC809和UBC886组合构建余甘子DNA指纹图谱。【结果】9条ISSR核心引物对28份余甘子样品扩增共得到686条条带,其中多态性条带667条,多态率为97.23%。通过聚类分析将28份余甘子样品聚为4类,其中大果油甘和甜油甘5号遗传分化较大,各单独聚为一类,珍珠油甘、甜油甘2号和甜油甘4号聚为一类,其余23个品种聚为一类。利用核心引物UBC809和UBC886组合,成功构建了余甘子DNA指纹图谱,供试28份余甘子品种(系)每个都有一套唯一的指纹图谱编码。【结论】成功构建了28份余甘子种质的指纹图谱,可用于余甘子种质资源的分类与鉴定,同时可用于余甘子杂交新品种选育。

【Objective】The genetic diversity of 28 emblica(Phyllanthus emblica L.)germplasm resources in South China collected by Guangdong Academy of Agricultural Sciences was analyzed by ISSR molecular marker technique and fingerprints were constructed.【Method】Nine pairs of core primers were screened from 96 pairs of ISSR primers for molecular markers of emblica and the genetic diversity of emblica germplasm resources was analyzed by UPGMA clustering.The DNA fingerprints were constructed according to primers UBC809 and UBC886.【Result】Nine pairs of ISSR core primers amplified 28 samples of emblica with a total of 686 bands and 667 polymorphic bands with a polymorphic ratio of 97.23%.Through cluster analysis,28 samples were clustered into 4 categories.Big fruit emblica and Sweet emblica No.5 had large genetic differentiation and were clustered into separate group.Pearl emblica,Sweet emblica No.2 and Sweet emblica No.4 were grouped together,and the remaining 23 varieties were grouped together.Combining the core primers of UBC809 and UBC886,the DNA fingerprints of emblica were successfully constructed.Each of the 28 varieties of emblica had one unique fingerprint coding.【Conclusion】The fingerprints constructed in this study can be used to classify and identify the germplasm resources of emblica,and for the breeding of new varieties.