高效液相色谱法测定辅食营养补充品中维生素B1含量

Determination of Vitamin B in Complementary Food Supplements by HPLC

采用高效液相色谱法检测辅食营养补充品中维生素B1的含量。样品在稀盐酸介质中恒温水解、中和后酶解,水解液用活性人造沸石净化,净化液用碱性铁氰化钾溶液衍生,正丁醇萃取后经C18反相色谱柱分离,用高效液相色谱-荧光检测器检测,外标法定量。通过优化前处理方式调整衍生液浓度以及样品净化的方式,本方法标准曲线相关系数≥0.999,加标回收率在95.2%~99.0%,保留时间稳定且重复性好,RSD值为1.0%,衍生液浓度在40~60 g·mL-1,结果显示本方法适用于检测维生素B1含量较高的辅食营养补充品,减少杂质干扰结果准确度高。

The content of vitamin B1 in supplementary food supplements was determined by HPLC. The sample was hydrolyzed at constant temperature in dilute hydrochloric acid medium, neutralized and then enzymolysis. The hydrolysate was purified by active artificial zeolite, and the purification solution was derived from basic potassium ferricyanide solution. After extraction with n-butanol, it was separated by C18 reversed-phase chromatographic column, detected by HPLC-fluorescence detector, and quantified by external standard method. The correlation coefficient of standard curve was ≥ 0.999, the recovery rate was 95.2% ~ 99.0%, the RSD value was 1.0%, and the concentration of derivative solution was in the range of 40 ~ 60 g·mL-1 Less impurity interference and high accuracy.