祛痘抗衰纳米乳凝胶的制备及其对兔耳痤疮模型的疗效观察

Preparation of anti acne nanoemulsion gel and its effect on rabbit ear acne model

[目的]制备含有秦皮甲素、光甘草定、白藜芦醇3种药效成分的纳米乳凝胶,并考察制得的纳米乳凝胶对兔耳痤疮模型的治疗效果。[方法]采用滴加水法制备纳米乳,HPLC法检测纳米乳中各有效成分的含量。用涂抹油酸的方法建立兔耳痤疮模型,组织切片确定模型建立成功,将建模成功的新西兰大白兔分为含药纳米乳凝胶组、空白纳米乳凝胶组、阳性对照Va组及模型组,分别给药7天,观察各组痤疮的治疗效果。[结果]成功制备了含有秦皮甲素、白藜芦醇、光甘草定三种药效成分的纳米乳,该纳米乳的平均粒径为(11.5±0.26)nm,PDI为0.195±0.004,Zeta电位为(-11.9±0.1)mV,纳米乳中秦皮甲素、白藜芦醇、光甘草定的包封率分别为(91.53±0.72)%、(92.85±0.37)%与(91.26±1.15)%,直观与HE染色结果显示该凝胶对兔耳痤疮模型有良好的治疗效果。[结论]秦皮甲素、光甘草定及白藜芦醇组分配伍制备的纳米乳凝胶对痤疮有良好的治疗作用。

[Objective]To prepare nanoemulsion gel by combining esculin,glbridin and resveratrol and to observe the therapeutic effect of rabbit ear acne model.[Methods]Nanoemulsion was prepared by dropwise addition and 3 active components were determined by HPLC.Oleic acid was used to establish a rabbit ear model of acne.HE staining was performed to confirm the success of acne modeling.The rabbit were divided into nanoemulsion gel group,blank nanoemulsion gel group,VA group,saline group.After seven days,observe the therapeutic effect of every group.[Results]The nanoemulsion gel containing esculin,glbridin and resveratrol was prepared successfully.The average size of the nanoemulsion was(11.5±0.26)nm,PDI was 0.195±0.004 and Zeta potential was(-11.9±0.1)mV.The encapsulation efficiency of esculin,resveratrol and glbridin were(91.53±0.72)%、(92.85±0.37)%and(91.26±1.15)%respectively.The results of naked eye observation and HE staining showed that the gel had good effect on rabbit ear acne model.[Conclusion]The nanoemulsion gel prepared by the combination of esculin,glbridin and resveratrold component has a good therapeutic effect on acne.