E-钙黏蛋白和血管内皮生长因子在胃肠间质瘤组织中的表达研究

Expressions of E-cadherin and vascular endothelial growth factor in gastrointestinal stromal tumors and their clinical significances

目的检测E-钙黏蛋白(E-cad)和血管内皮生长因子(VEGF)在胃肠间质瘤(GIST)组织中的表达并分析其与GIST患者临床病理特征的关系。方法收集青岛大学附属烟台毓璜顶医院2015年1月至2018年3月期间手术切除的40例GIST标本,应用免疫组织化学方法检测GIST组织中E-cad和VEGF蛋白表达。结果 40例GIST标本中E-cad和VEGF蛋白表达阳性率分别为10.0%(4/40)和50.0%(20/40)。E-cad和VEGF蛋白表达与均GIST患者的肿瘤直径、核分裂数及危险度分级有关(P<0.05),且E-cad蛋白阳性表达与VEGF蛋白阳性表达呈明显的负相关关系(rs=–0.55,P=0.001)。结论从本研究结果看,VEGF和E-cad与GIST的发生及发展可能有一定的关系,其可能可以作为GIST恶性分级评价的潜在指标,E-cad与VEGF的联合检测可能有助于指导GIST的临床治疗。

Objective To detect expressions of E-cadherin(E-cad)and vascular endothelial growth factor(VEGF)in gastrointestinal stromal tumor(GIST)tissues and analyze their relationships with clinicopathologic features of patients with GIST.Methods Forty paraffin-embeded specimens of surgical resected GIST from January 2015 to March 2018 in the Pathology Department of Yuhuangding Hospital Affilicated to Qingdao University were retrieved.The expressions of E-cad and VEGF proteins were detected by the immunohistochemical method.Results The positive expression rates of E-cad and VEGF proteins in the GIST tissues were 10.0%(4/40)and 50.0%(20/40),respectively.The positive expression rates of E-cad and VEGF proteins were associated with the tumor diameter,mitotic counts,and risk classification(P<0.05).The positive expression rate of the E-cad was negatively related to that of the VEGF in the GIST tissues(rs=–0.55,P=0.001).Conclusion From results of this study,VEGF and E-cad might be related with malignancy of GIST,which might be potential facators in predicting prognosis of GIST.